ELASTICITY OF SWOLLEN MICELLES FOR CHARACTERIZING VESICLES AND CELL MEMBRANES

用于表征囊泡和细胞膜的溶胀胶束的弹性

• 批准号: 6455792
• 负责人: MATTHEW S EDWARDS
• 金额: $ 8.4万
• 依托单位: SPELMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6455792
• 关键词: alkanes; amphiphilicity; atomic force microscopy; birefringences; cell membrane; elasticity; emulsions; fluidity; mathematical model; membrane activity; membrane model; micelles; nanotechnology; vesicle /vacuole; water solution

Elasticity is an important property of both animate and inanimate objects, covering their rigidity (flexibility) and structure survival. In both types of objects, this property is far from being completely understood, in fundamental ways. For animal structures, this concern is particularly vital and especially critical in maintaining the essence of life. Elasticity is intricately involved, in pathological diseases, and in the intrusion through and fluidity of cellular membranes, on the microscopic level. Also, some features, in the mechanism of aging, is implied in the analysis. Elasticity physics, of microemulsions (complex fluids of swollen micelles originating from the admixture of water and a surfactant in oil environment), -vesicles, and -cellular membranes, is essentially the same. Therefore, elastic information, about one of these structures, has implications for the others. Because of their simplicity and electro-optical behavior, as shown by us and others, microemulsions will be used as test materials to measure elasticity of vesicles and cell membranes. Specifically, we will: (1) use the Electro-Optical Birefringent (EOB) Kerr coefficient method to measure, in different aliphatic oil environments and at different temperatures, the Kerr co-efficient dependence on swollen- micellar droplet size omega (molar ratio of the number of water molecules to the number of surfactant molecules); (2) modify using the Mean Field Theory, Numerical Analysis, and the Calculus of Variations the single isolated droplet model; and (3) reconsider the data obtained in #1, above, based on the modified theory for the advantages of one model over the other. These research efforts will impact directly on the health of mankind by providing information on pathological diseases and the essential deterioration of cell membrane-liked structures. Our measurements and modeling developments are essential for subsequent-direct characterization of cellular membranes.

弹性是动画和无生命物体的重要特性，涵盖了它们的刚性（灵活性）和结构存活。在这两种类型的对象中，这种属性远非完全以基本方式理解。对于动物结构，这种关注特别重要，对于维持生命本质而言尤其重要。在微观水平上，弹性错综复杂地参与了病理疾病以及细胞膜的流动性和流动性。同样，分析中暗示了某些特征，在衰老机理中。微乳液的弹性物理学（源自水和石油环境中水和表面活性剂的肿胀胶束的复杂流体）， - 秒和 - 细胞膜，基本相同。因此，关于这些结构之一的弹性信息对其他结构具有影响。如我们和其他人所示，由于它们的简单性和电流行为，微乳液将用作测试材料，以测量囊泡和细胞膜的弹性。具体来说，我们将：（1）使用电 - 光谱（EOB）KERR系数方法在不同的脂肪族油环境中测量，在不同的温度下，Kerr的同时依赖于swollen-micellar-micellar滴液滴大小欧米茄（摩尔分子的摩尔比率摩尔分子的数量与表面表面活性分子的数量）; （2）使用平均场理论，数值分析和变异的计算进行修改，单个分离的液滴模型； （3）基于一个模型比另一个模型的优点的修改理论重新考虑上面的＃1获得的数据。这些研究工作将通过提供有关病理疾病的信息和细胞膜纽约结构的基本恶化，直接影响人类的健康。我们的测量和建模发展对于随后的细胞膜的直接表征至关重要。