GENETIC/MOLECULAR CONTROL OF ARABIDOPSIS FLOWER DEVELOPMENT

拟南芥花发育的遗传/分子控制

• 批准号: 6430871
• 负责人: JACK OKAMURO
• 金额: $ 28.24万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6430871
• 关键词: Arabidopsis; cytogenetics; developmental genetics; gene expression; homeobox genes; in situ hybridization; laboratory rabbit; light microscopy; molecular cloning; nucleic acid sequence; phenotype; plant genetics; plant growth /development; plant growth regulators; protein structure function; restriction fragment length polymorphism; restriction mapping; scanning electron microscopy

Little is known about the cell and molecule processes that control flower development and reproduction in plants. Over the past several years genetic and molecular studies in the model plant system Arabidopsis thaliana have defined a hierarchy of genes that control flower development. The homeotic gene APETALA2 (AP2) has been shown to play a central role in regulating the production of the floral meristem and in controlling floral organ identity. However, the molecular function of AP2 is not known. Recently, we cloned the AP2 gene and showed that it encodes a novel protein distinguished by the AP2-domain, a 68 amino acid repeated motif that is essential for AP2 activity. Currently our research efforts are focused on understanding the function of AP2 in the plant cell. In addition, we recently made significant progress towards characterizing a newly discovered relationship between the plant growth regulator gibberellic acid and the mutant phenotypes of the Arabidopsis floral homeotic mutants apetala2, apetala1, leafy and agamous. During the next four years we plan to address the following questions: 1) What is the molecular function of AP2 and AP2-like proteins in the plant cell? 2) What are the cofactors, target genes, and regulators of AP2 that together determine floral organ identity? 3) What is the relationship between gibberellic acid and the regulation of floral homeotic gene expression in Arabidopsis? Any of these questions would make a good thesis project for an MBRS graduate student thesis. Several more defined genetic and molecular projects will be given to undergraduate students, e.g., the cloning and isolation of mutant ap2 genes, the RFLP mapping of cloned AP2-like genes, and the structural analysis of floral homeotic mutants using light and scanning electron microscopy.

人们对控制花的细胞和分子过程知之甚少 植物的发育和繁殖。 过去几年 模式植物系统拟南芥的遗传和分子研究 拟南芥定义了控制花的基因层次结构 发展。 同源异型基因 APETALA2 (AP2) 已被证明发挥着 在调节花分生组织的产生和在 控制花器官的同一性。 然而，分子功能 AP2 未知。 最近，我们克隆了AP2基因并表明它 编码一种以 AP2 结构域为特征的新型蛋白质，该结构域由 68 个氨基酸组成 AP2 活性所必需的重复基序。 目前我们的 研究工作的重点是了解 AP2 在 植物细胞。 此外，我们最近在以下方面取得了重大进展： 表征新发现的植物生长之间的关系 调节剂赤霉酸和拟南芥突变体表型 花同源突变体 apetala2、apetala1、叶状和无性。 在接下来的四年里，我们计划解决以下问题： 1) AP2和AP2样蛋白在细胞中的分子功能是什么 植物细胞？ 2）什么是辅因子、靶基因和调节因子？ AP2 共同决定花器官身份？ 3）什么是 赤霉酸与花调控的关系 拟南芥中的同源基因表达？ 任何这些问题都会 为 MBRS 研究生论文制定一个好的论文项目。 一些 将给予更明确的遗传和分子项目 本科生，例如突变体ap2的克隆和分离 基因、克隆的 AP2 样基因的 RFLP 作图以及结构 使用光和扫描电子分析花同源突变体 显微镜。