Structure of HIV-1 Vpu Membrane Protein by NMR

通过 NMR 分析 HIV-1 Vpu 膜蛋白的结构

• 批准号: 6550936
• 负责人: ANTHONY A MRSE
• 金额: $ 3.66万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6550936
• 关键词: bioimaging /biomedical imaging; genome; high performance liquid chromatography; human immunodeficiency virus 1; lipid bilayer membrane; magnetic resonance imaging; membrane proteins; method development; nuclear magnetic resonance spectroscopy; protein structure function; structural biology; virus protein

DESCRIPTION (provided by applicant): Vpu is a small (81 residue) membrane protein encoded in the genome of HPV-I. Because of its small size and integral function in the HIV-1 lifecycle, NMR structural studies of Vpu can contribute to the development of membrane protein structure elucidation methods while simultaneously pursuing answers to AIDS-related research questions. The focus of this study is to develop NMR methods for determining the structures of membrane proteins. Specifically, the goals are to: 1. Determine the structure of the membrane protein Vpu; and 2. Utilize the structural information to describe its biological functions. Solid-state NMR spectroscopy will be performed on oriented lipid bilayer samples of Vpu. These studies will be compared to those obtained from complementary techniques, including solution-state NMR on micelle samples and solid-state MAS NMR on unoriented bilayer samples.

描述（由申请人提供）：VPU是在HPV-I基因组中编码的小（81个残基）膜蛋白。由于其在HIV-1生命周期中的大小和整体功能，VPU的NMR结构研究可以有助于膜蛋白结构阐明方法的发展，同时同时追求与AIDS相关的研究问题的答案。这项研究的重点是开发用于确定膜蛋白结构的NMR方法。具体而言，目标是：1。确定膜蛋白VPU的结构； 2。利用结构信息来描述其生物学功能。 固态NMR光谱将在VPU的定向脂质双层样品上进行。将将这些研究与从互补技术获得的研究进行比较，包括胶束样品上的溶液状态NMR和无定向双层样品的固态MAS NMR。