Dissection of the mitotic checkpoint component BUBR1

有丝分裂检查点组件 BUBR1 的解剖

• 批准号: 6551668
• 负责人: Yinghui Mao
• 金额: $ 3.83万
• 依托单位: LUDWIG INSTITUTE FOR CANCER RES LTD
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6551668
• 关键词: Xenopus oocyte; biological signal transduction; cell cycle; centromere; chromosomes; enzyme activity; intermolecular interaction; kinesin; microtubules; mitotic spindle apparatus; phosphotransferases; postdoctoral investigator; protein protein interaction; protein structure function

DESCRIPTION (provided by applicant): Eukaryotic organisms have evolved a "mitotic checkpoint" to monitor the metaphase-anaphase transition in order to assure high fidelity transmission of genetic information to daughter cells. This checkpoint prevents progression through mitosis prior to attachment of every centromere to microtubules of the mitotic spindle. In attempting to identify how checkpoint signaling is linked to microtubule attachment, earlier work from the Cleveland lab has proposed that the centromere- bound microtubule motor CENP-E is one link which provides microtubule attachment, relaying this to the centromere-bound checkpoint kinase BUBR1. The focus of this proposal is to test this model for CENP-E involvement in linking the vertebrate checkpoint to microtubule attachment, focusing on the ability to manipulate the cell cycle in vitro (using Xenopus egg extracts).

描述（由申请人提供）： 真核生物已经进化了一个“有丝分裂检查点”，以监测中期 - 解相体的转变，以确保将遗传信息的高忠诚传播给子细胞。该检查点可以通过有丝分裂来防止在每个丝粒附着在有丝分裂主轴的微管上的情况下进展。为了确定检查点信号如何链接到微管附件时，克利夫兰实验室的早期工作提出，Centromere-Bound-Bound-Boundibule Microtule Motor CENP-E是一个链接，它提供了微管附件，将其转发至中心粒结合结合的检查点Kinase Kinase Kinase kinase kinase bubr1。该提案的重点是测试该模型的CENP-E参与，以将脊椎动物检查点与微管附着联系起来，重点是在体外操纵细胞周期的能力（使用Xenopus Egg提取物）。