V1 to V2 Projections in Normal Vision and Amblyopia

正常视力和弱视的 V1 到 V2 投影

• 批准号: 6525092
• 负责人: LAWRENCE C SINCICH
• 金额: $ 4.62万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6525092
• 关键词: Macaca; amblyopia; autoradiography; behavioral /social science research tag; brain mapping; cell population study; cytochrome oxidase; enzyme activity; eye coordination disorder; neural information processing; neuroanatomy; radiotracer; sensory deprivation; visual cortex; visual perception

In primates, visual information passes from the primary visual area (V1) to the second visual area (V2) before distribution to higher cortical areas. An accurate description of the projections linking V 1 and V2 is crucial for understanding how the brain deciphers visual images. Prior studies have shown that V2 is partitioned into three compartments, known as pale, thin, and thick stripes, defined by their content of a metabolic enzyme called cytochrome oxidase (CO). Our principal goal is to describe the anatomical projections from V1 to each V2 stripe compartment. In Specific Aim #1 we will make injections of a retrograde tracer into single CO stripes in V2 of normal macaques. The resulting pattern of labeled cells in V1 will be correlated with the V2 stripe that received the injection. Our preliminary data indicate that, contrary to a previous report, layer 4B and interblobs both project to thick stripes and pale stripes. In Specific Aim #2 we will make paired injections of two different tracers into adjacent thick stripes and pale stripes to determine if different subpopulations of cells in layer 4B and interblobs project to these V2 compartments. In Specific Aim #3 we will make injections of [3H]proline into V1 to correlate patches of efferent projections with CO staining patterns in V2. In Specific Ai/s #4 we will examine the V1->V2 projections in animals raised with early monocular deprivation. These experiments will advance our knowledge of the mechanisms underlying amblyopia, an important cause of visual loss that affects 2% of the American population. We hypothesize that a selective loss of V1->V2 projections emanating from the ocular dominance columns serving the deprived eye contributes to the loss of vision in amblyopia.

在灵长类动物中，视觉信息从初级视觉区（V1）传递到第二视觉区（V2），然后再分布到更高的皮层区域。准确描述连接V1和V2的投射对于理解大脑如何解读视觉图像至关重要。先前的研究表明，V2被分为三个区室，称为苍白，薄和厚条纹，由其称为细胞色素氧化酶（CO）的代谢酶的含量定义。我们的主要目标是描述从V1到每个V2条纹室的解剖投影。在特定目标#1中，我们将逆行示踪剂注射到正常猕猴V2的单个CO条纹中。V1中标记细胞的结果图案将与接受注射的V2条纹相关。我们的初步数据表明，与以前的报告相反，层4 B和interblob都投影到厚条纹和浅色条纹。在特定目标#2中，我们将两种不同的示踪剂成对注射到相邻的厚条纹和浅条纹中，以确定层4 B和interblob中的不同细胞亚群是否投射到这些V2区室。在特定目标#3中，我们将向V1中注射[3 H]脯氨酸，以将传出投射斑块与V2中的CO染色模式相关联。在特定Ai/s#4中，我们将检查在早期单眼剥夺下饲养的动物的V1->V2投射。这些实验将推进我们对弱视机制的认识，弱视是影响2%美国人口的视力丧失的重要原因。我们假设，选择性丧失V1->V2投射从眼优势柱发出服务剥夺眼有助于弱视的视力丧失。