Nuclear Localization of DNA-Binding Polyamides

DNA 结合聚酰胺的核定位

• 批准号: 6487436
• 负责人: PABLO D DAVIDOV
• 金额: $ 2.37万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6487436
• 关键词: DNA binding protein; DNA footprinting; amides; cell biology; cell line; cell membrane; chimeric proteins; confocal scanning microscopy; gel electrophoresis; membrane permeability; membrane transport proteins; molecular shape; nuclear membrane; nucleic acid sequence; peptide chemical synthesis; postdoctoral investigator

The fellowship proposal is targeted at the synthesis and assay of a number of DNA-binding hairpin polyamides tethered to a "cellular transporter". These conjugate molecules not only are expected to permeate the cellular membrane but are also projected to localize in the nucleus of the cell where the target DNA lies. The target molecules will be synthesized using well-established peptide synthesis protocols and solid-phase synthesis. The conjugate molecules will be assayed versus ten cell lines with confocal microscopy. The success of this project would have wide ranging implications for further in vivo experiments involving small molecules that bind DNA sequence-specifically with affinities comparable to nature's proteins.

奖学金提案针对的是束缚在“细胞转运蛋白”上的许多DNA结合发夹的合成和测定。这些结合分子不仅有望渗透到细胞膜，而且预计将定位在靶DNA所在的细胞的细胞核中。将使用良好的肽合成方案和固相合成来合成靶分子。结合分子将被测定与共聚焦显微镜的十个细胞系进行测定。该项目的成功将对进一步的体内实验具有广泛的影响，这些实验涉及与自然蛋白相媲美的小分子特异性结合的DNA序列。