Development of an Optimal cDNA Array
最佳 cDNA 阵列的开发
基本信息
- 批准号:6525819
- 负责人:
- 金额:$ 1.78万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-08-10 至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION: (Applicant?s Description) One of the most exciting applications
for arrays so far is the monitoring of gene expression (mRNA). Presently, mRNA
abundance is determined by hybridizing short or long probes to arrays that are
composed of either short sequences of cDNA, long open reading frames of cDNA,
or synthesized oligonucleotides. Our objective is to determine what microarray
system would give the most accurate and meaningful mRNA abundance information.
Known limitations to current schemes prompt us to propose new approaches that
will be compared systematically to attain the goal of developing a microarray
with the strongest and most reproducible signal representative of the
transcription being monitored. Two important questions must be addressed. What
type of target link gives the strongest and reproducible signal? Which type of
target and probe, long or short, will give the truest representation of each
gene? In order to accomplish our goal, the surface and coupling chemistry that
give the most immobilized and accessible probe will be determined. The length
of probe cDNA that accurately represents an mRNA of interest must also be
identified. The working hypothesis is that longer pieces of cDNA will most
accurately represent an mRNA because s the length increases there will be less
homology between the sequence. Finally, it will be determined if the strongest,
most specific hybridization signal is given by the optimal target or a
digestion of the optimal target. The model systems parameters will then be used
to create an array capable of monitoring mRNA of biological interest, to
validate the reproducibility and dependability of our model. This work is
innovative because if one microarray could be shown to be superior, scientists
can work within one "operating system" to allow communication of expression
data in a relevant and meaningful fashion between different experiments and
different laboratories.
描述:(申请人?s描述)最令人兴奋的应用之一
基因表达(mRNA)的监测。目前,mRNA
通过将短或长探针与
由cDNA的短序列,cDNA的长开放阅读框,
或合成的寡核苷酸。我们的目标是确定微阵列
系统将提供最准确和最有意义的mRNA丰度信息。
现有方案的已知局限性促使我们提出新的方法,
将系统地进行比较,以达到开发微阵列的目标
具有最强和最可再现的信号代表
转录被监控。必须解决两个重要问题。什么
哪种类型的目标链路给出最强和可再现的信号?哪种类型的
目标和探测器,长或短,将给出每个目标和探测器的最真实的表示。
吉恩?为了实现我们的目标,表面和耦合化学,
给出最固定化的和可接近的探针。长度
准确代表感兴趣的mRNA的探针cDNA也必须
鉴定工作假设是,较长的cDNA片段将大多数
因为当长度增加时,
序列之间的同源性。最后,将决定是否最强,
最特异性的杂交信号由最佳靶或
最佳目标的消化。然后将使用模型系统参数
为了产生能够监测生物学感兴趣的mRNA的阵列,
验证了模型的可重复性和可靠性。这项工作是
创新是因为如果一个微阵列可以被证明是上级的,
可以在一个“操作系统”内工作,
不同实验之间的相关和有意义的数据,
不同的实验室
项目成果
期刊论文数量(0)
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会议论文数量(0)
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