Improved DNA Polymerases for Genomic Sequencing

用于基因组测序的改进 DNA 聚合酶

• 批准号: 6583877
• 负责人: Thomas William Schoenfeld
• 金额: $ 9.95万
• 依托单位: LUCIGEN CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-27 至 2004-03-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6583877
• 关键词: DNA directed DNA polymerase; bacterial virus; biochemical evolution; method development; molecular cloning; nucleic acid sequence; protein purification; thermophilic organism

DESCRIPTION (provided by applicant): This technology is intended to reduce the costs of sequencing by improving the ability to sequence through difficult templates such as G/C or A/T rich regions, direct and inverted repeats, homopolymer tracts, and di, and tri-nucleotide repeats. This will decrease finishing costs by reducing gaps. Additional improvements will enable direct sequencing of colony derived plasmids and more robust sequencing of BACs, cosmids and genomic DNA. These objectives will be attained by examining an entirely new class of DNA polymerases as improved sequencing reagents.

描述(由申请人提供)：这项技术旨在通过提高通过困难模板如G/C或A/T富集区、正向和反向重复序列、均聚体区域、二核苷酸重复序列和三核苷酸重复序列进行测序的能力来降低测序成本。这将通过减少差距来降低整理成本。进一步的改进将使菌落衍生的质粒直接测序成为可能，并使BAC、宇宙染色体和基因组DNA的测序更加可靠。这些目标将通过检测一种全新的DNA聚合酶作为改进的测序试剂来实现。

项目成果

2,2'-(Disulfanedi-yl)dibenzoic acid-N,N'-bis-(4-pyridyl-meth-yl)ethane-dithio-amide (1/1).

2,2-(二硫烷二基)二苯甲酸-N,N-双-(4-吡啶基-甲基)乙烷-二硫代酰胺(1/1)。

• DOI: 10.1107/s1600536810036755
• 发表时间: 2010
• 期刊: Acta crystallographica. Section E, Structure reports online
• 作者: Arman,HadiD;Miller,Tyler;Poplaukhin,Pavel;Tiekink,EdwardRT
• 通讯作者: Tiekink,EdwardRT

Genome signature analysis of thermal virus metagenomes reveals Archaea and thermophilic signatures.

• DOI: 10.1186/1471-2164-9-420
• 发表时间: 2008-09-17
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Pride DT;Schoenfeld T
• 通讯作者: Schoenfeld T