Oriented Protein Microarray for Functional Studies

描述(由申请人提供)：提出了一种新的方法 利用金属配位化学和电子探针技术制备蛋白质微阵列 多组氨酸标记的蛋白质分子。建议的方法旨在 具有高度选择性和易于连接的化学成分的微阵列，具有均匀的 和可控制的固定化蛋白质分子的取向，并与 蛋白质处于天然状态，很容易被溶液中的目标访问。 利用6x-His终止绿色荧光蛋白的初步实验 (GFP)证明了拟议方法的可行性和潜力。我们 将确立定向蛋白质微阵列的优势 随机取向阵列上蛋白质-配体相互作用的研究 现有的方法。我们还建议探索蛋白质固定化而不是 高选择性金属离子表面的提纯。 建议的商业应用：不可用

DESCRIPTION (provided by applicant):A novel method is proposed for the fabrication of protein microarrays using metal coordination chemistry and poly-histidine tagged protein molecules. The proposed method is aimed at microarrays with highly selective and facile linking chemistry, with uniform and controllable orientation of immobilized protein molecules, and with proteins in native states and easily accessible by targets in the solution. Preliminary experiments using 6x-His terminated green-fluorescence protein (GFP) demonstrated the feasibility and potential of the proposed approach. We will establish the advantage of an oriented protein microarray in protein-ligand interaction studies over randomly oriented arrays fabricated by existing methods. We also propose to explore protein immobilization without purification on the highly selective metal ion surface. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE