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The Influence of Collagen Structure and Surface Biochemistry on Cell Function and Angiogenesis

胶原蛋白结构和表面生物化学对细胞功能和血管生成的影响

基本信息

批准号：
1937226
负责人：
金额：
--
依托单位：
University of Cambridge
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1937226
关键词：
Influence Collagen Structure Surface Biochemistry

项目摘要

This project will comprise a systematic study of these challenges with regard to angiogenesis in ice templated scaffolds for bone regeneration, exploring the interplay between surface biochemistry and material structure to achieve an optimised cellular response.The initial step of this project involves the design and fabrication of ice templated scaffolds. The effect of mould design, directly applied heat sources and heat sinks will be used to tailor the scaffold structure. Ice nucleation will be controlled by the selective introduction of nucleation sites at specific mould positions to control pore structure and directionality. The scaffold architecture will be visualised and characterised by SEM and micro-CT for understanding as well as adjusting the key parameters such as pore size, interconnectivity and homogeneity of scaffold architecture with regard to the aimed application. Relevant in-vitro cell culture techniques will be used to assess the influence of the structural parameters on cell growth and migration.Secondly, a co-culture system with an appropriate ratio of osteoblasts and endothelial cells needs to be established in order to vascularise the produced scaffolds. A suitable assessment of the relationship between length and diameter of formed capillaries and the structural key parameters of scaffolds will be carried out. Finally, the influence of EDC treatment of the scaffolds on angiogenesis and cell function will be evaluated to establish an optimal cross-linking level which allows improved angiogenesis and provides appropriate structural stability of the scaffolds.

该项目将对用于骨再生的冰模板支架中血管生成的这些挑战进行系统研究，探索表面生物化学和材料结构之间的相互作用，以实现优化的细胞反应。该项目的第一步涉及冰模板支架的设计和制造。模具设计、直接应用的热源和散热器的效果将用于定制脚手架结构。冰成核将通过在特定模具位置选择性引入成核位点来控制孔结构和方向性。支架结构将通过 SEM 和微型 CT 进行可视化和表征，以了解和调整关键参数，例如支架结构的孔径、互连性和均匀性，以满足目标应用的需要。相关的体外细胞培养技术将用于评估结构参数对细胞生长和迁移的影响。其次，需要建立具有适当比例的成骨细胞和内皮细胞的共培养系统，以使产生的支架血管化。对形成的毛细血管的长度和直径与支架的结构关键参数之间的关系进行适当的评估。最后，将评估支架的 EDC 处理对血管生成和细胞功能的影响，以建立最佳交联水平，从而改善血管生成并提供支架适当的结构稳定性。