Nanofluidics-based DNA Screening Device

基于纳米流体的 DNA 筛选装置

• 批准号: 6444518
• 负责人: STEPHEN WHITFIELD TURNER
• 金额: $ 19.98万
• 依托单位: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444518
• 关键词: DNA; biomedical equipment development; biotechnology; fluid flow; genetic polymorphism; genetic screening; genome; nanotechnology; point mutation

DESCRIPTION (provided by applicant): A nanofluidic DNA screening device for cancer-related diagnosis and research will be developed. Point mutations and single nucleotide polymorphisms will be detected optically using fluorescent hybridization probes on a fully stretched strand of chromosomal DNA as it passes through a nanoscale tube. Array-based methods for detecting single-base differences have been demonstrated, but the fluidics method proposed here has several advantages, including immunity to coincidental sequence repeats, superior detection of rare mutations in mixed samples, and the retention of correlations between different loci on the same chromosome. Fluidics-based methods for screening genetic content have been studied before (Austin 1999), but have been limited by the methods of fluidics fabrication employed. For this device, a sacrificial layer technique for fabricating fluidics has been developed which allows precise control of complex fluid circuits with nanoscale dimensions (Turner 1998). A tube smaller than the persistence length of double-stranded DNA will prevent the molecule from folding back on itself while passing through the tube, thus eliminating the primary source of error in previous attempts to implement this method. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（由申请人提供）：一种纳米流体DNA筛选装置， 将开展与癌症有关的诊断和研究。点突变和 单核苷酸多态性将使用荧光标记进行光学检测。 杂交探针在染色体DNA的完全伸展链上， 通过一个纳米管基于阵列的单碱基检测方法 差异已被证明，但这里提出的射流方法， 几个优点，包括对重合序列重复的免疫力， 混合样本中罕见突变的上级检测， 同一条染色体上不同位点之间的相关性。基于流体的 以前已经研究了筛选遗传内容的方法（Austin 1999）， 但是受到所采用的射流制造方法的限制。为此 设备，用于制造流体的牺牲层技术已经被 开发，允许精确控制复杂的流体回路， 尺寸（Turner 1998）。一个小于持续长度的管子 双链DNA将防止分子折叠回自身， 通过管，从而消除误差的主要来源， 以前尝试过这种方法。 拟议商业应用：不可用