Rapid Identification of Secreted of F tularensis Antigen

描述（由申请方提供）：细胞内细菌病原体在感染后分泌蛋白质，其功能是改变宿主细胞的正常结构和代谢机制，从而促进其存活并避免宿主免疫监视。细胞内细菌病原体用于逃避或破坏宿主免疫系统的分子机制的最新发现将极大地促进抗菌疫苗和诊断工具的开发。与许多其他细胞内兼性病原体如鼠伤寒沙门氏菌、结核分枝杆菌和嗜肺军团菌一样，F.土拉热菌共享对巨噬细胞作为其优选宿主细胞的偏好。然而，与其他胞内细菌病原体相比，对F.土拉热菌在宿主细胞内部。我们已经设计并测试了一个系统，使我们能够识别I类可降解蛋白（CAP）在鼠伤寒沙门氏菌，并建议利用类似的方法研究F。土拉热。由于它们对宿主的加工和呈递途径的敏感性，CAP代表了设计和构建针对F的有效疫苗的潜在重要资源。土拉热。

DESCRIPTION (provided by applicant): Intracellular bacterial pathogens secrete proteins after infection that function to alter the normal structural and metabolic machinery of the host cell thus facilitating their survival and avoidance of host immune surveillance. Recent discoveries of the molecular mechanisms that intracellular bacterial pathogens use for evasion or subversion of the immune system of the host will greatly facilitate the development of antibacterial vaccines and diagnostic tools. Much like many other intracellular facultative pathogens such as Salmonella typhimurium, Mycobacterium tuberculosis and Legionella pneumophila, F. tularensis shares a predilection for macrophages as its preferred host cell. However, in contrast to other intracellular bacterial pathogens, little is known about virulence factors used by F. tularensis internally within its host cell. We have designed and tested a system that enables us to identify Class I Accessible Proteins (CAPs) in Salmonella typhimurium and propose to utilize a similar approach for the study of F. tularensis. Due to their susceptibility to the host' s processing and presentation pathways, CAPs represent a potentially important resource for the design and construction of effective vaccines against F. tularensis.