Rapid Identification of Secreted of F tularensis Antigen

土拉曲霉分泌抗原的快速鉴定

• 批准号: 6656274
• 负责人: FRED L HEFFRON
• 金额: $ 22.65万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-10 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6656274
• 关键词: Francisella tularensis; bacterial antigens; dendritic cells; epithelium; flow cytometry; gene expression; genetic library; laboratory mouse; macrophage; microarray technology; microorganism classification; technology /technique development; tularemia

DESCRIPTION (provided by applicant): Intracellular bacterial pathogens secrete proteins after infection that function to alter the normal structural and metabolic machinery of the host cell thus facilitating their survival and avoidance of host immune surveillance. Recent discoveries of the molecular mechanisms that intracellular bacterial pathogens use for evasion or subversion of the immune system of the host will greatly facilitate the development of antibacterial vaccines and diagnostic tools. Much like many other intracellular facultative pathogens such as Salmonella typhimurium, Mycobacterium tuberculosis and Legionella pneumophila, F. tularensis shares a predilection for macrophages as its preferred host cell. However, in contrast to other intracellular bacterial pathogens, little is known about virulence factors used by F. tularensis internally within its host cell. We have designed and tested a system that enables us to identify Class I Accessible Proteins (CAPs) in Salmonella typhimurium and propose to utilize a similar approach for the study of F. tularensis. Due to their susceptibility to the host' s processing and presentation pathways, CAPs represent a potentially important resource for the design and construction of effective vaccines against F. tularensis.

描述(申请人提供)：细胞内的细菌病原体在感染后分泌蛋白质，这些蛋白质的功能是改变宿主细胞的正常结构和代谢机制，从而促进它们的生存和避免宿主免疫监视。最近发现的细胞内细菌用来逃避或颠覆宿主免疫系统的分子机制将极大地促进抗菌疫苗和诊断工具的发展。就像许多其他细胞内兼性病原体，如鼠伤寒沙门氏菌、结核分枝杆菌和嗜肺军团菌一样，图拉氏杆菌有一个共同的偏好，即巨噬细胞是其首选宿主细胞。然而，与其他细胞内细菌病原体不同的是，图拉氏F菌在其宿主细胞内使用的毒力因子知之甚少。我们设计并测试了一种系统，使我们能够识别鼠伤寒沙门氏菌中的I类可访问蛋白(CAPS)，并建议使用类似的方法来研究图拉氏沙门氏菌。由于其对宿主S的加工和呈递途径的敏感性，CAPS对于设计和构建有效的图拉氏菌疫苗具有潜在的重要意义。