HLH GENE FUNCTION IN NEURONAL CELL DETERMINATION

描述（来自申请人摘要）：在果蝇中，神经元前体细胞 决定是由基本螺旋环抑制（bHLH）转录调控的 因素这些包括前神经bHLH激活蛋白，如Achaete、Scute 和《鱼鳞致命》Notch介导的侧抑制过程 信号通路抑制前神经基因表达。Notch信号是已知的 导致原神经基因的转录抑制。 该项目的目标是研究转录后调节 果蝇原神经基因Achaete、Scute和Lethal of Scute。的 研究人员提供的证据表明，Notch途径的激活也 导致Achaete前神经蛋白的翻译后抑制 通过其C-末端转录激活（TA）结构域。n末端 结构域也强烈抑制Achaete TA活性。Homoprotein C-和N-末端 结构域在Scute和L’Sc蛋白中高度保守， 其可能通过相同的途径进行转录后调节。

DESCRIPTION (from applicant's abstract): In Drosophila, neuronal precursor cell determination is regulated by basic Helix-Loop-Helix (bHLH) transcription factors. These include proneural bHLH activator proteins such as Achaete, Scute and Lethal of Scute. A lateral inhibition process mediated by the Notch signaling pathway inhibits proneural gene expression. Notch signaling is known to result in transcriptional repression of proneural genes. The goals of this project are to study the post-transcriptional regulation of the Drosophila proneural genes Achaete, Scute, and Lethal of Scute. The investigator provides evidence that activation of the Notch pathway also results in post-translational inhibition of the Achaete proneural protein through its C-terminal transcriptional activation (TA) domain. An N terminal domain also strongly inhibits Achaete TA activity. Homologous C- and N-terminal domains are highly conserved in the Scute and Lethal of Scute (L'Sc) proteins, which may be post-transcriptionally regulated by the same pathways.