HLH GENE FUNCTION IN NEURONAL CELL DETERMINATION

HLH 基因在神经细胞测定中的功能

• 批准号: 6529557
• 负责人: Michael A. Caudy
• 金额: $ 33.53万
• 依托单位: WINIFRED MASTERSON BURKE MED RES INST
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-08 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6529557
• 关键词: 3T3 cells; DNA binding protein; Drosophilidae; biological signal transduction; developmental genetics; developmental neurobiology; gel mobility shift assay; gene induction /repression; lethal genes; neurogenesis; neurogenetics; posttranslational modifications; protein purification; protein sequence; stem cells; transcription factor; transfection; yeast two hybrid system

DESCRIPTION (from applicant's abstract): In Drosophila, neuronal precursor cell determination is regulated by basic Helix-Loop-Helix (bHLH) transcription factors. These include proneural bHLH activator proteins such as Achaete, Scute and Lethal of Scute. A lateral inhibition process mediated by the Notch signaling pathway inhibits proneural gene expression. Notch signaling is known to result in transcriptional repression of proneural genes. The goals of this project are to study the post-transcriptional regulation of the Drosophila proneural genes Achaete, Scute, and Lethal of Scute. The investigator provides evidence that activation of the Notch pathway also results in post-translational inhibition of the Achaete proneural protein through its C-terminal transcriptional activation (TA) domain. An N terminal domain also strongly inhibits Achaete TA activity. Homologous C- and N-terminal domains are highly conserved in the Scute and Lethal of Scute (L'Sc) proteins, which may be post-transcriptionally regulated by the same pathways.

描述（来自申请人摘要）：在果蝇中，神经元前体细胞 决定是由基本螺旋环抑制（bHLH）转录调控的 因素这些包括前神经bHLH激活蛋白，如Achaete、Scute 和《鱼鳞致命》Notch介导的侧抑制过程 信号通路抑制前神经基因表达。Notch信号是已知的 导致原神经基因的转录抑制。 本项目的目标是研究转录后调控， 果蝇原神经基因Achaete、Scute和Lethal of Scute。的 研究人员提供的证据表明，Notch途径的激活也 导致Achaete前神经蛋白的翻译后抑制 通过其C-末端转录激活（TA）结构域。n末端 结构域也强烈抑制Achaete TA活性。Homoprotein C-和N-末端 结构域在Scute和L’Sc蛋白中高度保守， 其可能通过相同的途径进行转录后调节。