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microRNA regulation of virus gene expression

microRNA调控病毒基因表达

基本信息

批准号：
1939469
负责人：
金额：
--
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1939469
关键词：
microRNA regulation virus gene expression

项目摘要

Background. MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression at a post-transcriptional level. miRNAs play a central role in regulating a wide variety of biological processes and as such dysregulation of miRNA expression has been implicated in many human diseases. We have exciting preliminary data, using miRNA profiling, suggesting that host miRNA expression can be dysregulated during the KSHV lytic replication cycle. Therefore, miRNA dysregulation contributes a key role in mediating viral replication, pathogenesis and tumourigenesis.Objectives. The aim of this project is to determine how virus-mediated manipulation of host cell miRNAs regulates both virus and host cell gene expression during virus infection. Moreover, the project has potential to identify novel host cell restriction factors which prevent herpesvirus replication, highlighting potential therapeutic approaches for the treatment of this important family of viruses.Novelty. This project is highly novel. An emerging area of research suggests that manipulation of miRNA expression can have fundamental effects on host cell gene expression. The project will therefore investigate novel mechanisms utilised by the virus to regulate host cell gene expression which can dictate how the virus and host cell transcriptome is regulated to benefit virus replication.Timeliness. The project is timely as this is an emerging area of research. miRNAs are key regulators of gene expression and aberrant expression of miRNAs is increasingly being associated with human disease.Experimental Approach. The project utilises a multidisiplinary approach and cutting-edge methodology including miRNA seq, bioinformatic analysis, imaging and cell biology.

背景资料。MicroRNAs(MiRNAs)是一种在转录后水平调节基因表达的非编码短小RNA。MiRNAs在调节多种生物过程中起着核心作用，因此，miRNA表达的失调与许多人类疾病有关。我们有令人兴奋的初步数据，使用miRNA图谱，表明宿主miRNA的表达在KSHV裂解复制周期中可能受到失调。因此，miRNA的异常调节在介导病毒复制、发病机制和肿瘤发生中起着关键作用。本项目的目的是确定病毒介导的宿主细胞miRNAs在病毒感染过程中如何调节病毒和宿主细胞基因的表达。此外，该项目有可能确定防止疱疹病毒复制的新的宿主细胞限制因素，突出治疗这一重要病毒家族的潜在治疗方法。这个项目非常新颖。一个新兴的研究领域表明，对miRNA表达的操纵可以对宿主细胞的基因表达产生根本影响。因此，该项目将研究病毒用来调节宿主细胞基因表达的新机制，这种机制可以决定病毒和宿主细胞转录组如何调节以利于病毒复制。这个项目是及时的，因为这是一个新兴的研究领域。MiRNAs是基因表达的关键调节因子，miRNAs的异常表达与人类疾病的关系日益密切。该项目采用了多学科方法和尖端方法，包括miRNA序列、生物信息学分析、成像和细胞生物学。