CORE--CYTOMETRY FACILITY

核心——细胞计数设施

• 批准号: 6444620
• 负责人: DONALD BELLGRAU
• 金额: $ 25万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444620
• 关键词: animal tissue; biomedical facility; diabetes mellitus; flow cytometry

Analysis of individual cells and cell populations at the spatial, temporal and functional level lies at the heart of modern immunology, cell biology and developmental biology. The Cytometry Core focuses on the provision of light microscopal or fluorescence activated cell sorting (FACS) technologies which can be applied in combination with specific molecular probes such as antibodies, DNA, MHC-tetramers, intracellular reporter molecules to address fundamental questions relating to the pathogenesis, treatment and complications of type 1 and 2 diabetes. The Core will be located at the Barbara Davis Center and manned by 2 FTE technical staff under the director ship of Dr. D. Bellgrau. It will use items of large equipment recently obtained from NIH and charitable funding. Twelve services will be offered including standard FACS analyses and immunohistochemistry of solid tissues and more sophisticated analyses of signal transduction and subcellular events in living cells. Formal training will be offered without charge on a biweekly to monthly basis and expert consultation will be available for each of the services provided.

在空间、时间和功能水平上对单个细胞和细胞群进行分析是现代免疫学、细胞生物学和发育生物学的核心。细胞计数核心专注于提供光学显微镜或荧光激活细胞分选 (FACS) 技术，该技术可与抗体、DNA、MHC 四聚体、细胞内报告分子等特定分子探针结合使用，以解决与 1 型和 2 型糖尿病的发病机制、治疗和并发症相关的基本问题。核心将位于 Barbara Davis 中心，由 2 名 FTE 技术人员管理，由 D. Bellgrau 博士领导。它将使用最近从美国国立卫生研究院和慈善基金获得的大型设备。将提供 12 项服务，包括标准 FACS 分析和实体组织的免疫组织化学，以及对活细胞中信号转导和亚细胞事件的更复杂的分析。将每两周至每月免费提供正式培训，并为所提供的每项服务提供专家咨询。