CORE--CELL SCIENCE/FACS FACILITY

说明：（直接取自应用程序） 一大批糖尿病相关的研究调查人员在大学， 马萨诸塞州医学中心依赖于使用细胞培养和相关 技术.细胞科学核心（CSC）设施满足以下需求： 调查人员通过提供四个主要方面的服务：1）各种 常规细胞培养服务; 2）已建立DERC细胞库， 为参与者提供各种细胞系用于他们的工作; 3）一些 专业的免疫细胞培养服务，包括淋巴细胞分离， 以及刺激和产生单克隆抗体;以及4）细胞分选， 我们的荧光激活细胞分选仪（FACS）设备配备了 FACSCAN、FACSCalibur和双激光FACSTAR Plus以及三光束FACS Vantage。CSC位于重建和装备的 由医疗中心提供的跨部门实验室综合体，以及 与糖尿病有关的研究是其活动的一个重要组成部分。 设施包括用于细胞培养的单独房间，每个房间都配备有 使用二氧化碳组织培养箱和层流罩。中心实验室 区域内有程序控制的细胞冷冻机离心机液氮 用于细胞储存的冰箱和用于制备培养基的设备。 荧光激活细胞分选仪位于两个独立的，特别是 建造房间。组织培养服务包括培养基制备和 无菌检查、原代培养物制备、维护和 连续细胞系的分布和细胞的冷冻保存。的FACS 设备用于定量分析和分离细胞。这一核心 提高糖尿病研究的生产力：a）提供服务， 可在单个实验室中获得; B）允许研究者专注于 他们的特殊项目不需要开发细胞库，细胞分选， 能力和其他设施在自己的实验室;和c）通过 鼓励共享资源的调查人员之间的互动， 核心设施。

Description: (Taken directly from the application) A large number of diabetes-related research investigators at the University of Massachusetts Medical Center depend upon the use of cell culture and associated techniques. The Cell Science Core (CSC) Facility satisfies the needs of investigators by providing services in four major areas: 1) a variety of routine cell culture services; 2) a DERC cell bank has been established to provide participants with a variety of cell lines for their work; 3) a number of specialized immune cell culture services, including lymphocyte separation, and stimulation and production of monoclonal antibodies; and 4) cell sorting in our fluorescence activated cell sorters (FACS) Facility equipped with an FACSCAN, FACSCalibur and a Dual Laser FACSTAR Plus, and triple beam FACS Vantage. The CSC is located within the reconstructed and equipped interdepartmental laboratory complex provided by the Medical Center, and diabetes-related research is a significant component of its activity. Facilities include separate rooms for cell culture, each of which is equipped with C02 tissue culture incubators and laminar flow hoods. A central laboratory area contains a programmed cell freezer, centrifuges, liquid nitrogen refrigerators for cell storage, and equipment for preparation of culture media. Fluorescence activated cell sorters are located in two separate, specially constructed rooms. Tissue culture services include media preparation and sterility testing, preparation of primary cultures, maintenance and distribution of continuous cell lines, and cryopreservation of cells. The FACS facility is used for quantitatively analyzing and separating cells. This Core enhances the productivity of diabetes research by: a) providing services not available in individual laboratories; b) allowing investigators to focus on their particular projects without the need to develop cell banks, cell sorting capabilities, and other facilities in their own laboratories; and c) by encouraging interactions between investigators who share the resources of this Core facility.