CORE--CELL SCIENCE/FACS FACILITY

描述：(直接取自应用程序) 密歇根大学的大量糖尿病相关研究人员 马萨诸塞州医学中心依赖于使用细胞培养和相关 技巧。细胞科学核心(CSC)设施满足以下需求 通过在四个主要领域提供服务来调查：1)各种 常规细胞培养服务；2)建立了DERC细胞库，以 为参与者的工作提供各种细胞系；3)数量 专门的免疫细胞培养服务，包括淋巴细胞分离， 单抗的刺激和产生；4)细胞分选 我们的荧光激活细胞分类器(FACS)设施配备了 FACSCAN、FACSCalibur和双激光FACSTAR Plus以及三光束FACS 优势。CSC位于改建并配备了 由医学中心提供的跨部门实验室综合体，以及 与糖尿病相关的研究是其活动的重要组成部分。 设施包括单独的细胞培养房间，每个房间都配备了 配有二氧化碳组织培养箱和层流流罩。一个中心实验室 区域内有程控细胞冷冻机、离心机、液氮 用于细胞存储的冰箱，以及用于制备培养基料的设备。 荧光激活细胞分选器位于两个独立的，特别是 建造的房间。组织培养服务包括媒体准备和 无菌检测、原代培养准备、维护和 连续细胞系的分布和细胞的冷冻保存。《FACS》 该设备用于对细胞进行定量分析和分离。此核心 通过以下方式提高糖尿病研究的生产力：a)提供服务而不是 在个别实验室提供；b)使调查人员能够专注于 他们的特定项目无需开发细胞库、细胞分选 能力和其他设施在其自己的实验室中；和c) 鼓励共享这一资源的调查人员之间的互动 核心设施。

Description: (Taken directly from the application) A large number of diabetes-related research investigators at the University of Massachusetts Medical Center depend upon the use of cell culture and associated techniques. The Cell Science Core (CSC) Facility satisfies the needs of investigators by providing services in four major areas: 1) a variety of routine cell culture services; 2) a DERC cell bank has been established to provide participants with a variety of cell lines for their work; 3) a number of specialized immune cell culture services, including lymphocyte separation, and stimulation and production of monoclonal antibodies; and 4) cell sorting in our fluorescence activated cell sorters (FACS) Facility equipped with an FACSCAN, FACSCalibur and a Dual Laser FACSTAR Plus, and triple beam FACS Vantage. The CSC is located within the reconstructed and equipped interdepartmental laboratory complex provided by the Medical Center, and diabetes-related research is a significant component of its activity. Facilities include separate rooms for cell culture, each of which is equipped with C02 tissue culture incubators and laminar flow hoods. A central laboratory area contains a programmed cell freezer, centrifuges, liquid nitrogen refrigerators for cell storage, and equipment for preparation of culture media. Fluorescence activated cell sorters are located in two separate, specially constructed rooms. Tissue culture services include media preparation and sterility testing, preparation of primary cultures, maintenance and distribution of continuous cell lines, and cryopreservation of cells. The FACS facility is used for quantitatively analyzing and separating cells. This Core enhances the productivity of diabetes research by: a) providing services not available in individual laboratories; b) allowing investigators to focus on their particular projects without the need to develop cell banks, cell sorting capabilities, and other facilities in their own laboratories; and c) by encouraging interactions between investigators who share the resources of this Core facility.