CAMPYLOBACTER

弯曲杆菌

• 批准号: 6588841
• 负责人: GUILLERMO RUIZ-PALACIOS
• 金额: $ 17.24万
• 依托单位: EASTERN VIRGINIA MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6588841
• 关键词: CHO cells; Helicobacter; Mexico; bacteria infection mechanism; breast feeding; cell adhesion; clinical research; clinical trials; diarrhea; gastrointestinal epithelium; genetically modified animals; glycolipids; glycoproteins; hexosyltransferase; human milk; human subject; infant human (0-1 year); laboratory mouse; nutrition related tag; passive immunization; receptor binding; virulence

Campylobacter is one of the most common cause of diarrhea worldwide. Human milk protects young infants against Campylobacter diarrhea by several factors including fucosylated oligosaccharides. However, there is a lack of understanding of the chemical structure of these components and their mechanism of interaction with Campylobacter virulence traits associated with attachment to gut mucosa. The specific aims of this project are: 1) define the virulence markers of Campylobacter associated with attachment to epithelial cells; 2) characterize and purify milk glycoconjugates that inhibit Campylobacter infection; 3) assess passive protection in breast- fed children and experimentally in transgenic mice carrying a human fucoslytransferase gene expressed mainly in mammary tissue; 4) develop strategies for large-scale synthesis of fucosylated glycoconjugates active against Campylobacter infection; and 5) conduct clinical trials on safety and protection of fucosylated glycoconjugates against Campylobacter diarrhea. The long-term goal of this project is to develop methods for prevention and treatment of Campylobacter infection through understanding at the cellular and molecular level the mechanism of protection conferred by human milk against Campylobacter infection.

弯曲杆菌是世界范围内引起腹泻的最常见原因之一。人类 牛奶通过以下几种方式保护幼儿免受弯曲菌腹泻 包括岩藻糖化低聚糖在内的因素。然而，有一点是缺乏的 了解这些成分的化学结构和它们的 与弯曲杆菌毒力性状相互作用的机制 附着在肠粘膜上。本项目的具体目标是：1) 确定与附着相关的弯曲杆菌毒力标志物 对上皮细胞；2)表征和纯化乳糖偶联物 抑制弯曲杆菌感染；3)评估乳房的被动保护- 喂养儿童并在携带人类的转基因小鼠中进行实验 岩藻糖基转移酶基因主要在乳腺组织中表达；4)发育 岩藻糖基活性糖偶联物的大规模合成策略 抗弯曲杆菌感染；以及5)进行安全性临床试验 岩藻糖化糖偶联物对弯曲杆菌的保护作用 拉肚子。该项目的长期目标是开发用于 通过了解预防和治疗弯曲杆菌感染 在细胞和分子水平上，保护机制被赋予 通过母乳抗弯曲杆菌感染。