CORE--ANATOMY

核心--解剖学

• 批准号: 6581879
• 负责人: HANNAH C KINNEY
• 金额: $ 23.61万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581879
• 关键词: biomedical facility; brain stem; computer data analysis; histology; human tissue; infant human (0-1 year); neuroanatomy; neurochemistry; newborn animals; postmortem; swine

Description: Core B is the anatomy core which is physically divided with one facility at Children?s Hospital, directed by Dr Kinney and a satellite facility at Dartmouth Medical School, directed by Dr Filiano. Dr Kinney?s Laboratory has been equipped and functional for some time. and is well organized to do the studies proposed. She has requested some updates for the MCID system for 3-D autoradiography, and funding for a separate PC workstation in the institutional Image Graphics Laboratory. The technical work of cutting sections and making autoradiographs and immunocytochemistry will be performed by MS Rava in the Children?s Hospital Core. She will be assisted by a second full time histotechnologist whose major responsibility will be in the construction of three dimensional representation of the brain stem, in the Image graphics laboratory. A post doctoral fellow will assist in various function of the core I the preparation and slides and in the imaging and analysis of data. There was no explanation of the facilities which will be required for the immunocytochemical studies but no additional monies were requested and are assumed to be available. The satellite facility at Dartmouth Medical school will be developed and equipped for brain morphologic analysis. Equipment has been requested for this; a cryostat, a fluorescent microscope, imaging digitizing computer, a camera lucida, a microscope booth, a refrigerator and a freezer. These items will provide a good facility. One histotechnician will prepare the tissues of 215 piglets per year for identification of the sites of physiology experimentation, of ten piglets for Neurotensin and camera lucida studies, and of 5 animals for serial brain stem anatomic studies of piglet arcuate homologue. In this lab the 20 piglets will be prepared for study by autoradiographic and immunocytochemical studies in the Boston Core.

描述：核心B是解剖核心，在物理上分为一个 儿童设施？由Kinney博士和一颗卫星指挥的 达特茅斯医学院的设施，由菲利亚诺博士指导。肯尼博士S 实验室已配备并运作了一段时间。并且很好地 组织进行所提出的研究。她要求提供一些最新情况， 用于三维放射自显影的MCID系统，并为单独的PC工作站提供资金 在机构图像图形实验室。 切片和放射自显影的技术工作， 免疫细胞化学将由MS Rava在儿童中进行？医院 核心她将由第二名全职组织技术专家协助， 主要责任将在建设三维 脑干的代表性，在图像图形实验室。一个帖子 博士研究员将协助核心I的各项功能的准备工作 和幻灯片以及成像和数据分析。 没有解释将需要的设施， 免疫细胞化学研究，但没有要求额外的资金，并且 假设是可用的。 达特茅斯医学院的卫星设施将得到发展， 进行脑形态学分析已要求提供设备， 这个;低温恒温器、荧光显微镜、成像数字化计算机、 照相机，显微镜室，冰箱和冰柜。这些项目 将提供良好的设施。一名组织技术员将准备 每年215头仔猪，用于鉴定生理部位 实验，10只小猪的神经降压素和照相机的研究，和 对5只猪的脑干进行了连续的解剖学研究 同源物在该实验室中，将通过以下方式准备20头仔猪用于研究： 放射自显影和免疫细胞化学研究。