Murine Model of Gammaherpesvirus Latency
伽马疱疹病毒潜伏期小鼠模型
基本信息
- 批准号:6514926
- 负责人:
- 金额:$ 26.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-07-01 至 2006-06-30
- 项目状态:已结题
- 来源:
- 关键词:B lymphocyte Burkitt's lymphoma Epstein Barr virus Kaposi's sarcoma dendritic cells disease /disorder model gene induction /repression host organism interaction human herpesvirus 8 laboratory mouse latent virus infection macrophage microorganism immunology polymerase chain reaction recombinant virus southern blotting virus genetics
项目摘要
DESCRIPTION (provided by applicant): The long-term objective of the proposed
research is to define the mechanisms that enable the gamma herpesviruses to
persist indefinitely within their immunocompetent hosts. These mechanisms
underlie the pathogenesis and oncogenic potential associated with these
viruses, particularly in individuals with acquired or inherited
immunodeficiencies. Unfortunately, due to the extreme host restrictions of the
human gamma-herpesviruses Epstein-Barr virus (EBV) and Kaposi's
sarcoma-associated herpesvirus (KSHV), it has not been possible to directly
address the mechanisms responsible for EBV and KSHV persistence in a relevant
host setting. Infection of laboratory mice with the murine gamma-herpesvirus
MHV-68 (closely related to KSHV) offers a highly tractable and potential model
of human gamma-herpesvirus persistence, but viral latency in this system has
not been sufficiently characterized at the molecular level to enable
exploitation of this model for these purposes. Therefore, the immediate goals
of the proposed research are to define the molecular characteristics of MHV-68
latency. To obtain our objective, we propose three specific aims. Under Aim 1
we will define the programs of MHV-68 latency gene expression during the
establishment and maintenance phases of latency within the hematopoietic-cell
reservoirs of latent virus (activated and resting B cells, macrophages, and
dendritic cells). Specifically, we will screen these cells by RT-PCR and
Southern blot hybridization for expression of the MHV-68 latency genes M2, M3,
M1 1, 72, 73 and 74. Additionally, we propose to identify potentially novel
MHV-68 latency genes expressed in these cells that are not apparent from
analysis of the viral genornic DNA sequence. Under Aim 2 we will address the
functions of three latency-associated genes: M2, 73 and 74, for which little or
no information is available. Under Aim 3 we will define the impact of MHV-68
latency on cellular gene expression in vivo at the cellular level. Together,
these specific aims will fill the significant gaps that currently remain in our
knowledge of the latent life cycle of MHV-68, as well as provide better insight
into the mechanisms that the gamma-herpesviruses as a group exploit to maintain
latency, and thus contribute to their pathogenic potential.
描述(由申请人提供):建议的长期目标
项目成果
期刊论文数量(0)
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专利数量(0)
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{{ truncateString('JEFFERY T SAMPLE', 18)}}的其他基金
Viral Long Noncoding RNA Functions in Epstein-Barr Virus Infection
病毒长非编码 RNA 在 Epstein-Barr 病毒感染中的功能
- 批准号:
8806522 - 财政年份:2014
- 资助金额:
$ 26.19万 - 项目类别:
Viral Long Noncoding RNA Functions in Epstein-Barr Virus Infection
病毒长非编码 RNA 在 Epstein-Barr 病毒感染中的功能
- 批准号:
8659714 - 财政年份:2014
- 资助金额:
$ 26.19万 - 项目类别:
Viral Long Noncoding RNA Functions in Epstein-Barr Virus Infection
病毒长非编码 RNA 在 Epstein-Barr 病毒感染中的功能
- 批准号:
9017925 - 财政年份:2014
- 资助金额:
$ 26.19万 - 项目类别:
Mechanisms of Gene Regulation by EBV EBNA-1 Protein
EBV EBNA-1蛋白的基因调控机制
- 批准号:
7621316 - 财政年份:2009
- 资助金额:
$ 26.19万 - 项目类别:
Mechanisms of Gene Regulation by EBV EBNA-1 Protein
EBV EBNA-1蛋白的基因调控机制
- 批准号:
7847575 - 财政年份:2009
- 资助金额:
$ 26.19万 - 项目类别:
Mechanisms of Gene Regulation by EBV EBNA-1 Protein
EBV EBNA-1蛋白的基因调控机制
- 批准号:
7681398 - 财政年份:2008
- 资助金额:
$ 26.19万 - 项目类别:
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