C11 ACETATE PET IMAGING OF PROSTATE AND RENAL CANCER

描述：(改编自PI的摘要)需要新的方法来 前列腺癌和肾癌的代谢成像。正电子发射断层扫描 (PET)允许横断面代谢成像，现在正在成为一种 重要的临床诊断影像方式。糖酵解的PET成像 使用示踪剂氟脱氧葡萄糖的新陈代谢已被证明在 几种恶性肿瘤的诊断和分期，但在 肾癌和前列腺癌。描绘代谢途径的正电子放射性示踪剂 除了糖酵解代谢外，还需要其他因素来充分发挥 肿瘤科的宠物医生。碳-11醋酸酯是一种潜在的放射性示踪剂 恶性肿瘤中间代谢的改变。目的 这项研究是为了了解参与代谢的相关途径 在PET上观察到醋酸盐放射性示踪剂的高摄取率和保留率 某些恶性肿瘤的影像检查，尤其是肾细胞癌和 前列腺癌。醋酸盐是中间代谢的中心分子， 可以同时进入分解代谢和合成代谢途径。在这项拟议的研究中， 醋酸酯放射性示踪剂的参与代谢途径和中间库 将利用细胞培养和动物模型在体外研究选定的 恶性肿瘤和池的组织培养将与 中间体和合成代谢相关酶的表达测定 了解醋酸脂代谢的限速步骤和优势 放射性示踪剂醋酸酯在体内的摄取和滞留 某些恶性肿瘤。~(11)C-醋酸酯在人体正电子发射计算机断层成像中的动力学 前列腺癌和肾癌切除前和显微手术的受试者 携带特定恶性肿瘤异种移植的无瘤小鼠的PET成像将是 对这些组织的体外酶测定进行分析和比较 确定转运和中间代谢酶表达的作用 在肾癌和前列腺癌的示踪动力学中。碳-11醋酸酯 前列腺癌和肾癌受试者的影像研究 这项研究还将与外科手术和 组织病理学检查以评估临床诊断的准确性。

DESCRIPTION: (Adapted from PI's Summary) There is a need for new approaches to metabolic imaging of prostate and renal cancer. Positron emission tomography (PET) allows for cross sectional metabolic imaging, and is now emerging as an important clinical diagnostic imaging modality. PET imaging of glycolytic metabolism using the tracer fluorodeoxyglucose has proven useful in the diagnosis and staging of several malignancies, but has shown limited value in renal and prostate cancer. Positron radiotracers depicting metabolic pathways other than glycolytic metabolism are needed to fully develop the potential of PET in oncology. Carbon-11 acetate is a potential radiotracer for the depiction of the altered intermediary metabolism in malignant neoplasms. The purpose of this research is to understand the participating metabolic pathways responsible for the high uptake and retention of acetate radiotracer observed on PET imaging in certain malignant neoplasms, most notably renal cell carcinoma and prostate cancer. Acetate is a central molecule of intermediary metabolism with access to both catabolic and anabolic pathways. In this proposed research, the participating metabolic pathways and intermediate pools of acetate radiotracer will be investigated in vitro using cell culture and animal models of selected malignant neoplasms and tissue culture of pools will be correlated with measurements of the expression of enzymes involved in intermediary and anabolic lipid metabolism of acetate to understand the rate limiting steps and dominant tracer pools responsible for the uptake and retention of radiotracer acetate in certain malignancies. Kinetics of carbon-11 acetate on PET imaging of human subjects with prostate cancer and renal cancer prior to resection, and on micro PET imaging of athymic mice bearing xenographs of selected malignancies will be analyzed and compared to enzyme measurement of these tissues in vitro to determine the role of transport and intermediary metabolism enzyme expression in the tracer kinetics of renal and prostate cancer. The carbon-11 acetate imaging of human subjects with prostate cancer and renal cell carcinoma involved in this study will also be compared with surgical and histopathological findings to assess diagnostic accuracy in clinical diagnosis.