Core--Animal model support

（申请人的摘要）动物模型支持核心将有助于 资源，标准化的协议和试剂，以合作研究 进行了深入的调查核心是由S。Alex Mitsialis博士 该核心将负责以下具体职能： 向参与合作研究实验的SCOR调查员提供 通过管理特定小鼠的扩增， 系，建立转基因系之间的杂交，并通过对转基因系进行基因分型， 后代，如有需要。(b)保持氧循环仪的环境氧气控制 室，并使用它们将动物暴露于定义的低氧和高氧下， 实验方案，以确保一致性和再现性， 涉及这两种损伤模型的研究，以及（c）设计并产生DNA 用于分析小鼠肺损伤和炎症的微阵列， 支持SCOR调查人员将这项技术应用于 小鼠慢性肺组织差异基因表达的系统分析 疾病模型

(Applicant's Abstract) The Animal Model Support Core will contribute resources, standardized protocols and reagents to collaborative studies between SCOR investigators. The Core is directed by S. Alex Mitsialis, Ph.D. This Core will be responsible for the following specific functions: (a) Supply to SCOR investigators engaged in collaborative studies experimental animals from all four mouse models by managing the expansion of specific mouse lines, setting up crosses between transgenic lines and by genotyping the progeny, as needed. (b) Maintain the Oxycycler environmental oxygen control chambers and use them to expose animals to hypoxia and hyperoxia under defined experimental protocols, in order to insure consistency and reproducibility in studies involving these two injury models, and (c) Design and produce a DNA microarray intended to analyze lung injury and inflammation in the mouse and support the SCOR investigators in applying this technology towards a systematic analysis of differential gene expression in the mouse chronic lung disease models.