Core--Animal model support

核心--动物模型支持

• 批准号: 7108693
• 负责人: S ALEX MITSIALIS
• 金额: $ 29.56万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7108693
• 关键词: animal colony; biomedical facility; bronchopulmonary dysplasia; disease /disorder model; genetically modified animals; hyperoxia; hypoxia; laboratory mouse; microarray technology

(Applicant's Abstract) The Animal Model Support Core will contribute resources, standardized protocols and reagents to collaborative studies between SCOR investigators. The Core is directed by S. Alex Mitsialis, Ph.D. This Core will be responsible for the following specific functions: (a) Supply to SCOR investigators engaged in collaborative studies experimental animals from all four mouse models by managing the expansion of specific mouse lines, setting up crosses between transgenic lines and by genotyping the progeny, as needed. (b) Maintain the Oxycycler environmental oxygen control chambers and use them to expose animals to hypoxia and hyperoxia under defined experimental protocols, in order to insure consistency and reproducibility in studies involving these two injury models, and (c) Design and produce a DNA microarray intended to analyze lung injury and inflammation in the mouse and support the SCOR investigators in applying this technology towards a systematic analysis of differential gene expression in the mouse chronic lung disease models.

(申请者摘要)动物模型支持核心将做出贡献 协作研究的资源、标准化方案和试剂 在SCOR调查人员之间。《核心》由S·亚历克斯·米西亚里斯博士执导。 该核心将负责以下具体职能：(A) 向从事合作研究和实验的SCOR调查人员提供 通过管理特定鼠标的扩展，从所有四种小鼠模型中获取动物 品系，在转基因品系之间建立杂交，通过对 后代，根据需要。(B)保持氧循环器的环境氧气控制 并使用它们将动物暴露在规定的低氧和高氧环境中 实验协议，以确保一致性和重现性 涉及这两种损伤模型的研究，以及(C)设计和生产DNA 旨在分析小鼠肺损伤和炎症的微阵列 支持SCOR调查人员将这项技术应用于 小鼠慢性肺组织差异基因表达的系统分析 疾病模型。