CORE--GENERAL SCIENTIFIC

通用科学核心的目的是帮助研究人员在动物外科、免疫细胞化学、透射电子显微镜和图像分析方面满足项目教职员工的要求。核心还协助研究人员进行各种生化分析，以及分子生物学方法。核心设施包括开展拟议研究所需的人员和设备。这个核心将进行一般的动物准备，包括麻醉、气管切开、导尿术、体温监测和生理变量的调节。核心将协助研究人员为动物的创伤性脑损伤做好准备，如个别项目中所述。此外，还提供专业知识来修改未来研究可能需要的实验准备工作。核心人员将进行免疫细胞化学程序，以及开发新的程序，以可视化更多的抗体。核心将处理组织进行塑料嵌入，进行光学和电子显微镜分析。高质量的薄片将由核心内部人员制作，并进行染色以进行超微结构分析。已建立的图像分析程序将用于量化免疫反应细胞的频率，以及确定挫伤面积和体积。最后，核心将协助调查人员进行暗室程序以及制作照片幻灯片和照片。

The purpose of the General Scientific Core is to assist investigators in animal surgery, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core also assists investigators in various biochemical analyses, as well as molecular biological approaches. The Core facilities consists of personnel and equipment needed to perform the proposed studies. This Core will conduct general animal preparation, including anesthesia, tracheotomy, catheterization, temperature monitoring, and regulation of physiological variables. The Core will assist investigators in preparing the animals for traumatic brain injury, as described in the individual projects. In addition, expertise is available to modify experimental preparations that may be required for future investigations. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissues for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within the Core and stained for ultrastructural analysis. Established image analysis procedures will be utilized to quantitate the frequency of immunoreactive cells, as well as to determine contusion areas and volume. Finally, the Core will assist investigators in darkroom procedures and the generation of photographic slides and prints.