Core--Immunohistochemistry

核心--免疫组化

• 批准号: 6485256
• 负责人: GREGORY BOSSART
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485256
• 关键词: biomedical facility; chemical kinetics; environmental health; environmental toxicology; immunocytochemistry; marine toxins

Description (Taken from the Investigator's Abstract) The objectives of this core facility are to support three of the proposed projects in this study: Lovelace Institute Rat studies, the Sheep and Rat Animal Model Studies, and the Biomarker and Epidemiology Studies. This facility will provide a variety of services including immunohistochemical staining for toxins, characterization of cellular elements on swabs and scraping and review of histological slides. The immunohistochemistry Core Facility is located within the Division of Comparative Pathology in the Papanicolaou Cancer Research Building. The Division has over 6,000 square feet of laboratory, administrative and office space. There are four full time faculty members and an administrative and support staff of 10 individuals. The laboratories are fully equipped to perform the proposed studies except for the specialized microscope for morphometry, which is included in the budget. The complete histopathology laboratory is available including a Fischer Histomatic model 166MP Tissue Processor, a Shandon Embedding station, two Leitz model 1512 Microtomes, an AO Cryostat, and complete equipment for routine, special stains, and immunohistochemistry. In addition, basic research equipment such as centrifuges (refrigerated/non- refrigerated, and high speed), light microscopes, refrigerators, freezers, autoclaves, C02 incubators, biological safety cabinets, microfuges, ELISA readers, a gamma counter, fume hoods, water baths, fluorescent microscopes, and a walk-in cold room are also available. The shared department resource area also includes a fully equipped laboratory for PCR analysis. Office space is available for participants in this facility that includes their own personal computers with Internet access, multiple programs including Word and Excel, and several data analysis programs.

描述（摘自研究者摘要）该核心设施的目的是支持本研究中的三个拟议项目：Lovelace研究所大鼠研究，绵羊和大鼠动物模型研究以及生物标志物和流行病学研究。该设施将提供多种服务，包括毒素的免疫组织化学染色，拭子和刮擦上细胞成分的表征以及组织学载玻片的审查。免疫组织化学核心设施位于Papanicolaou癌症研究大楼的比较病理学部门内。该部门拥有超过6000平方英尺的实验室、行政和办公空间。有4名全职教师和10名行政和支持人员。除了包含在预算内的用于形态测定的专用显微镜外，实验室的设备齐全，可以进行拟议的研究。完整的组织病理学实验室包括一台Fischer组织模型166MP组织处理器，一台山东包埋站，两台Leitz模型1512显微切片机，一台AO低温恒温器，以及完整的常规、特殊染色和免疫组织化学设备。此外，基本的研究设备，如离心机（冷藏/非冷藏，高速），光学显微镜，冰箱，冰柜，高压灭菌器，二氧化碳培养箱，生物安全柜，微生物，ELISA阅读器，伽马计数器，通风柜，水浴，荧光显微镜，和步入式冷藏室也可用。共享部门资源区域还包括一个设备齐全的PCR分析实验室。该设施为参与者提供办公空间，包括可以上网的个人电脑，包括Word和Excel在内的多种程序以及一些数据分析程序。