Core--Immunohistochemistry

核心--免疫组化

• 批准号: 6564463
• 负责人: GREGORY BOSSART
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564463
• 关键词: biomedical facility; chemical kinetics; environmental health; environmental toxicology; immunocytochemistry; marine toxins

Description (Taken from the Investigator's Abstract) The objectives of this core facility are to support three of the proposed projects in this study: Lovelace Institute Rat studies, the Sheep and Rat Animal Model Studies, and the Biomarker and Epidemiology Studies. This facility will provide a variety of services including immunohistochemical staining for toxins, characterization of cellular elements on swabs and scraping and review of histological slides. The immunohistochemistry Core Facility is located within the Division of Comparative Pathology in the Papanicolaou Cancer Research Building. The Division has over 6,000 square feet of laboratory, administrative and office space. There are four full time faculty members and an administrative and support staff of 10 individuals. The laboratories are fully equipped to perform the proposed studies except for the specialized microscope for morphometry, which is included in the budget. The complete histopathology laboratory is available including a Fischer Histomatic model 166MP Tissue Processor, a Shandon Embedding station, two Leitz model 1512 Microtomes, an AO Cryostat, and complete equipment for routine, special stains, and immunohistochemistry. In addition, basic research equipment such as centrifuges (refrigerated/non- refrigerated, and high speed), light microscopes, refrigerators, freezers, autoclaves, C02 incubators, biological safety cabinets, microfuges, ELISA readers, a gamma counter, fume hoods, water baths, fluorescent microscopes, and a walk-in cold room are also available. The shared department resource area also includes a fully equipped laboratory for PCR analysis. Office space is available for participants in this facility that includes their own personal computers with Internet access, multiple programs including Word and Excel, and several data analysis programs.

描述（摘自研究者摘要）该核心设施的目标是支持本研究中的三个拟议项目：洛夫莱斯研究所大鼠研究、绵羊和大鼠动物模型研究以及生物标志物和流行病学研究。该设施将提供各种服务，包括毒素的免疫组织化学染色、拭子细胞成分的表征以及组织学载玻片的刮取和审查。免疫组化核心设施位于Papanicolaou癌症研究大楼的比较病理学部门内。该司拥有超过6，000平方英尺的实验室、行政和办公空间。有四名全职教师和10名行政和支持人员。除了预算中包括的用于形态测定的专用显微镜外，实验室都有进行拟议研究的充分设备。完整的组织病理学实验室包括Fischer Histomatic 166 MP型组织处理器、Shandon包埋站、两台Leitz 1512型切片机、AO冷冻恒温器以及用于常规、特殊染色和免疫组织化学的完整设备。此外，基础研究设备如离心机（冷藏/非冷藏和高速）、光学显微镜、冰箱、冷冻机、高压灭菌器、CO2培养箱、生物安全柜、微量离心器、ELISA读数器、γ计数器、通风橱、水浴、荧光显微镜和步入式冷藏室也是可用的。共享部门资源区还包括一个设备齐全的PCR分析实验室。该设施为参与者提供办公空间，包括他们自己的可上网的个人电脑、Word和Excel等多种程序以及若干数据分析程序。