Developing a perfusion culture bioreactor and 3D culture techniques to more accurately model cancer metastasis and tumour cell behaviour in vitro.

开发灌注培养生物反应器和 3D 培养技术，以更准确地模拟癌症转移和肿瘤细胞的体外行为。

• 批准号: 1944652
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1944652
• 关键词: Developing; perfusion; culture; bioreactor; 3D

Current 2D cell culture techniques poorly represent the native cellular environment of tumours cells whose conditions are known to influence a range of cellular behaviours including survival, metabolism and protein expression. As a consequence, culturing cells in this simplistic system may compromise the behavioural fidelity of responses achieved in vitro. With the aim of better recapitulating the conditions present in this locale, developing 3D culture systems and a perfusion bioreactor for cell culture experiments may garner cell responses that more accurately replicate and predict those in vivo.3D cultures allow cells to grow within a more natural 3D framework and enable researchers to establish concentration gradients across the growth environment, better mimicking the heterogeneity of local cellular conditions experienced in vivo. Moreover, a perfusion bioreactor would allow in vitro experiments to investigate the impact of blood flow, particular in modelling the metastatic capabilities of tumour cells migrating and subsequently invading secondary sites. Together, these would provide a powerful platform to investigate tumour cell behaviour and obtain results with greater relevance to in vivo models.

目前的二维细胞培养技术很难代表肿瘤细胞的天然细胞环境，已知肿瘤细胞的条件会影响一系列细胞行为，包括存活、代谢和蛋白质表达。因此，在这种简单的系统中培养细胞可能会损害在体外获得的反应的行为保真度。为了更好地概括该地区的情况，开发3D培养系统和用于细胞培养实验的灌注生物反应器可能会获得更准确地复制和预测体内细胞反应的细胞反应。3D培养允许细胞在更自然的3D框架内生长，并使研究人员能够在生长环境中建立浓度梯度，更好地模拟体内局部细胞条件的异质性。此外，灌注生物反应器将允许在体外实验中研究血流的影响，特别是在模拟肿瘤细胞迁移和随后入侵继发部位的转移能力方面。总之，这些将提供一个强大的平台来研究肿瘤细胞的行为，并获得与体内模型更相关的结果。