Biology/Immunology Of Corneal Epithelial Stem Cells

角膜上皮干细胞的生物学/免疫学

• 批准号: 6507406
• 负责人: ROBERT B. NUSSENBLATT
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6507406
• 关键词: antireceptor antibody; artificial immunosuppression; cell biology; combination therapy; cornea disorder; corneal epithelium; cytokine receptors; gene expression; genetically modified animals; immunogenetics; immunosuppressive; interleukin 2; laboratory mouse; laboratory rabbit; neural plasticity; neurogenetics; neuroimmunomodulation; nonhuman therapy evaluation; slow release drug; stem cell transplantation; stem cells; tissue /cell culture; transplant rejection

Currently two animal protocols (one rabbit and one mouse) are ongoing. The focus of the rabbit studies is to understand and prevent immunologic rejection of the transplanted limbal stem cells using donor tissue manipulation and local immunosuppression including sustained release implants. Studies currently in progress involve tissue engineering using cultured corneal epithelial cells for the treatment stem cell deficiency. These latter studies will likely provide the basis for future clinical studies using cultured corneal epithelial cells. The mouse studies will be focused on limbal stem cell biology and immunlogy taking advantage of various transgenic strains. Studies will also attempt to determine the long-term survival of transplantated limbal stem cells and the effect of systemic immunosuppression using mouse anti-IL2 receptor antibody. The plasticity of limbal stem cells in various stages of development will also be studied. Using gene expression studies, attempts will be made to determine the factors that are important for maintaining "stemness".This project began recently and there is no data to report

目前正在进行两项动物实验（一只兔子和一只老鼠）。兔研究的重点是通过供体组织操作和局部免疫抑制（包括缓释植入物）来了解和预防移植角膜缘干细胞的免疫排斥反应。目前正在进行的研究涉及组织工程，利用培养的角膜上皮细胞治疗干细胞缺乏症。后一项研究可能为将来使用培养的角膜上皮细胞进行临床研究提供基础。小鼠的研究将集中在角膜缘干细胞生物学和免疫学方面，利用各种转基因菌株。研究还将尝试确定移植的角膜缘干细胞的长期存活以及使用小鼠抗il - 2受体抗体进行全身免疫抑制的效果。我们还将研究角膜缘干细胞在不同发育阶段的可塑性。利用基因表达研究，将试图确定维持“干性”的重要因素。这个项目最近才开始，没有数据可以报告