Atypical DNA replication in the absence of origins in archaeon Haloferax volcanii

古细菌 Haloferax volcanii 中缺乏起源的非典型 DNA 复制

• 批准号: 1944984
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1944984
• 关键词: Atypical; DNA; replication; absence; origins

DNA replication is widely accepted to initiate at specific areas within the genome, known as 'origins of replication'. Whilst origins were previously thought to be essential for survival, deletion of all chromosomal origins of replication in archaeon Haloferax volcanii has no ill effect, with originless cells growing 7.5% faster than the wild type strain (Hawkins et al., 2013, Nature, doi:10.1038/nature12650). Initiation of replication is generally well understood: origin recognition proteins remodel DNA at the origin to a more open structure, allowing loading of replication components, which will form a functional 'replisome' that will replicate the DNA. Little is known about how DNA is replicated without origins; origins and origin recognition proteins are non-essential in H. volcanii, and thus an alternate mode of replication initiation must be occurring. This project aims to assess how the molecular mechanisms of replication are altered in cells lacking origins of replication. The components required for replication will be analysed and differences between origin-dependent and origin-independent replication will be assessed.

人们普遍认为 DNA 复制是在基因组内的特定区域（称为“复制起点”）开始的。虽然起源以前被认为对于生存至关重要，但在古细菌 Haloferax volcanii 中删除所有染色体复制起点并没有不良影响，无起源细胞的生长速度比野生型菌株快 7.5%（Hawkins 等，2013，Nature，doi：10.1038/nature12650）。复制的启动通常是众所周知的：起点识别蛋白将DNA在起点处重塑为更开放的结构，从而允许加载复制组件，这将形成能够复制DNA的功能性“复制体”。人们对DNA如何在没有起源的情况下复制知之甚少。起源和起源识别蛋白在H. volcanii中不是必需的，因此必须发生替代的复制起始模式。该项目旨在评估缺乏复制起点的细胞中复制的分子机制是如何改变的。将分析复制所需的组件，并评估起源依赖性复制和起源无关复制之间的差异。

项目成果

Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii.

• DOI: 10.1093/molbev/msy075
• 发表时间: 2018-08-01
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Ausiannikava D;Mitchell L;Marriott H;Smith V;Hawkins M;Makarova KS;Koonin EV;Nieduszynski CA;Allers T
• 通讯作者: Allers T