Application of novel Ms approaches to environmental sampling.

新颖的女士方法在环境采样中的应用。

• 批准号: 1945145
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1945145
• 关键词: Application; novel; Ms; approaches; environmental

The term 'molecular scatology' has normally been taken to mean the analysis of faecal DNA. Whilst this provides valuable information, there is considerable scope to extend the range of analyses, particularly to those that provide information at the phenotype level. The industrial partner, Waters Corporation (Micromass UK Ltd) are a world leading developer of advanced mass spectrometers, coupled with innovative 'front end' sources for sampling of a broad range of biological material. They are the sole supplier of the iKnife REIMS system that will be used in this programme. The student will complete part of their training at their state of the art R&D facilities in Wilmslow. The host institution and the CASE partner have already completed preliminary data that demonstrate the ease with which phenotypic data can be acquired, using a new sampling method (rapid evaporative ionisation mass spectrometry; REIMS) that can generate complex mass spectral phenotypes from each sample in a matter of seconds, with no sample preparation being needed.REIMS is the basis of the iKnife, a device that is based on analysis of the smoke produce by diathermy, the process whereby extreme heat is generated locally through high frequency voltages applied across a sample. The smoke that is generated has been demonstrated to have considerable chemical richness, and can be used to differentiate, for example, normal and tumour tissue. We have preliminary data that show that a REIMS-generated fingerprint can be used in multivariate analyses of rodent faecal material to differentiate groups of individuals, based on species or sex, and wish to consolidate these initial investigations using a REIMS installation in the labs of the academic partner, coupled to an advanced mass spectrometer.

“分子散射学”这个术语通常被认为是指对粪便DNA的分析。虽然这提供了有价值的信息，但有相当大的空间来扩大分析的范围，特别是那些提供表型水平信息的分析。该工业合作伙伴Waters Corporation(MicroMassUK Ltd)是先进质谱仪的世界领先开发商，并配备了用于对多种生物材料进行采样的创新“前端”来源。他们是iKnife Reims系统的唯一供应商，该系统将在本计划中使用。学生将在威尔姆斯洛的最先进的研发设施完成部分培训。主办机构和案例合作伙伴已经完成了初步数据，证明了使用一种新的采样方法(快速蒸发电离质谱仪；REIMS)可以很容易地获得表型数据，这种方法可以在几秒钟内从每个样本产生复杂的质谱型，而不需要准备样本。REIMS是iKnife的基础，iKnife是一种基于透热产生的烟雾的分析设备，通过对样本施加高频电压，局部产生极端热量。已经证明，产生的烟雾具有相当丰富的化学成分，可以用来区分正常组织和肿瘤组织。我们有初步数据表明，ReIMS生成的指纹可以用于啮齿动物粪便材料的多变量分析，以区分基于物种或性别的个体群体，并希望使用学术伙伴实验室中的Reims设备，加上先进的质谱仪来巩固这些初步调查。

项目成果

Rapid identification of mosquito species and age by mass spectrometric analysis.

• DOI: 10.1186/s12915-022-01508-8
• 发表时间: 2023-01-24
• 期刊: BMC BIOLOGY
• 影响因子: 5.4
• 作者: Wagner, Iris;Grigoraki, Linda;Enevoldson, Peter;Clarkson, Michael;Jones, Sam;Hurst, Jane L. L.;Beynon, Robert J. J.;Ranson, Hilary
• 通讯作者: Ranson, Hilary

Social status and ejaculate composition in the house mouse.

• DOI: 10.1098/rstb.2020.0083
• 发表时间: 2020-12-07
• 期刊: Philosophical transactions of the Royal Society of London. Series B, Biological sciences
• 作者: Bayram HL;Franco C;Brownridge P;Claydon AJ;Koch N;Hurst JL;Beynon RJ;Stockley P
• 通讯作者: Stockley P

The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species-a potential new tool in entomology.

• DOI: 10.1098/rsob.200196
• 发表时间: 2020-11
• 期刊: Open biology
• 影响因子: 5.8
• 作者: Wagner I;Koch NI;Sarsby J;White N;Price TAR;Jones S;Hurst JL;Beynon RJ
• 通讯作者: Beynon RJ