Rapid identification of mosquito species and age by mass spectrometric analysis.
Rapid identification of mosquito species and age by mass spectrometric analysis.
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DOI：
10.1186/s12915-022-01508-8
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发表时间：
2023-01-24
期刊：
影响因子：
5.4
通讯作者：
Ranson, Hilary
中科院分区：
文献类型：
--
作者：
Wagner, Iris;Grigoraki, Linda;Enevoldson, Peter;Clarkson, Michael;Jones, Sam;Hurst, Jane L. L.;Beynon, Robert J. J.;Ranson, Hilary
关键词：
在预测病原体传播风险和评估媒介控制干预措施对公众健康的影响方面，一种快速、准确的方法来识别和识别不同年龄段的蚊子种群将是一大进步。虽然其他光谱学或转录学方法显示出了希望，但目前的方法依赖于具有挑战性的形态学技术或简单的二进制分类，无法识别足够老到具有传染性的种群子集。在这项研究中，研究了快速蒸发电离质谱仪(REMS)鉴定实验室饲养的野生蚊子的种类和年龄的能力。基于主成分/线性判别分析(PC-LDA)的REMS对冈比亚按蚊复合体形态相同物种的识别准确率超过97%，基于随机森林分析的准确率超过84%。年龄划分为1天、5~6天、14~15天，准确率分别为99%(PC-LDA)和91%(随机林)。当对来自英国的野生蚊子进行测试时，Reims数据可以确定标本的种类和年龄，准确率分别为91%和90%。REMS在分辨按蚊种类和年龄方面的准确性可与红外光谱分析方法相媲美。与目前的基于解剖的方法相比，处理时间和易用性具有显著的优势。重要的是，当使用在不同环境条件下饲养的野生蚊子，以及当蚊子被冷冻或干燥储存时，准确度保持不变。因此，这种高通量方法有可能对媒介种群进行快速、实时的监测，为替代干预措施的影响提供昆虫学证据。网上版载有补充材料，可在10.1186/s12915-022-01508-8查阅。
A rapid, accurate method to identify and to age-grade mosquito populations would be a major advance in predicting the risk of pathogen transmission and evaluating the public health impact of vector control interventions. Whilst other spectrometric or transcriptomic methods show promise, current approaches rely on challenging morphological techniques or simple binary classifications that cannot identify the subset of the population old enough to be infectious. In this study, the ability of rapid evaporative ionisation mass spectrometry (REIMS) to identify the species and age of mosquitoes reared in the laboratory and derived from the wild was investigated. The accuracy of REIMS in identifying morphologically identical species of the Anopheles gambiae complex exceeded 97% using principal component/linear discriminant analysis (PC-LDA) and 84% based on random forest analysis. Age separation into 3 different age categories (1 day, 5–6 days, 14–15 days) was achieved with 99% (PC-LDA) and 91% (random forest) accuracy. When tested on wild mosquitoes from the UK, REIMS data could determine the species and age of the specimens with accuracies of 91 and 90% respectively. The accuracy of REIMS to resolve the species and age of Anopheles mosquitoes is comparable to that achieved by infrared spectroscopy approaches. The processing time and ease of use represent significant advantages over current, dissection-based methods. Importantly, the accuracy was maintained when using wild mosquitoes reared under differing environmental conditions, and when mosquitoes were stored frozen or desiccated. This high throughput approach thus has potential to conduct rapid, real-time monitoring of vector populations, providing entomological evidence of the impact of alternative interventions. The online version contains supplementary material available at 10.1186/s12915-022-01508-8.
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快速蒸发电离质谱法（REIMS）从念珠菌属内的培养物种鉴定提供了准确的直接直接。
影响因子：
4.6
作者：
Cameron SJ;Bolt F;Perdones-Montero A;Rickards T;Hardiman K;Abdolrasouli A;Burke A;Bodai Z;Karancsi T;Simon D;Schaffer R;Rebec M;Balog J;Takáts Z
通讯作者：
Takáts Z
影响因子：
3.7
作者：
Joy, Teresa K.;Gutierrez, Eileen H. Jeffrey;Riehle, Michael A.
通讯作者：
Riehle, Michael A.
DOI：
10.1126/science.1258524
发表时间：
2015-01-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fontaine MC;Pease JB;Steele A;Waterhouse RM;Neafsey DE;Sharakhov IV;Jiang X;Hall AB;Catteruccia F;Kakani E;Mitchell SN;Wu YC;Smith HA;Love RR;Lawniczak MK;Slotman MA;Emrich SJ;Hahn MW;Besansky NJ
通讯作者：
Besansky NJ
影响因子：
6.1
作者：
Balog, Julia;Perenyi, Dora;Takats, Zoltan
通讯作者：
Takats, Zoltan
影响因子：
17.1
作者：
Balog, Julia;Sasi-Szabo, Laszlo;Takats, Zoltan
通讯作者：
Takats, Zoltan