The Dystroglycan LINC

肌营养不良聚糖 LINC

• 批准号: 1945659
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1945659
• 关键词: Dystroglycan; LINC

Dystroglycan is an essential protein that is a linker through biological membranes including the plasma membrane and the nuclear membrane. This project will address fundamental aspects of nuclear structure and dynamics employing a multidisciplinary approach comprising molecular cell biology and advanced microscopy. The project will be supervised by two world leading experts in their fields: Professor Steve Winder in the Department of Biomedical Science who works on the cell biology of dystroglycan, and Professor Jamie Hobbs who is at the forefront of research advances using atomic force microscopy (AFM) to probe biological systems. The project will use genetic approaches in cells in tissue culture to manipulate the function of dystroglycan, a newly identified component of the nuclear membrane, and how it contributes to nuclear shape, nuclear resistance to stress and nuclear position in the cell. We will use biophysical approaches to measure nuclear deformation and stability and cell biological approaches to investigate the effects of altered nuclear deformation on cell migration in two and three dimensions, using microfluidic chambers. AFM and high resolution fluorescence microscopy, coupled with genetic manipulation will provide a powerful combination of approaches to understand the essential functions of dystroglycan in the nucleus.

肌营养不良聚糖是一种必需的蛋白质，其是通过生物膜（包括质膜和核膜）的接头。该项目将采用包括分子细胞生物学和先进显微镜在内的多学科方法来解决核结构和动力学的基本问题。该项目将由两个世界领先的专家在各自的领域监督：史蒂夫·温德教授在生物医学科学系谁在肌营养不良蛋白聚糖的细胞生物学工作，和教授杰米·霍布斯谁是在研究进展的前沿使用原子力显微镜（AFM）探测生物系统。该项目将在组织培养的细胞中使用遗传方法来操纵肌营养不良蛋白聚糖的功能，肌营养不良蛋白聚糖是一种新发现的核膜成分，以及它如何有助于细胞核的形状，细胞核对压力的抵抗力和细胞核的位置。我们将使用生物物理学方法来测量核变形和稳定性，并使用微流体室来研究改变的核变形对二维和三维细胞迁移的影响。原子力显微镜和高分辨率荧光显微镜，加上遗传操作将提供一个强大的组合的方法来了解细胞核中的肌营养不良蛋白聚糖的基本功能。