MHC Class I in the Generation of Autoimmune Diseases

MHC I 类在自身免疫性疾病产生中的作用

• 批准号: 6559073
• 负责人: DINAH SINGER
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6559073
• 关键词: MHC class I antigen; antigen presentation; antigen presenting cell; autoimmune disorder; cell population study; chemical structure function; disease /disorder etiology; disease /disorder model; gene expression; genetic regulation; genetically modified animals; hormone regulation /control mechanism; immunogenetics; laboratory mouse; molecular pathology; systemic lupus erythematosus; thyroglobulin; thyroid hormones; thyrotropin

MHC class I genes, which provide immune surveillance against intracellular pathogens, are dynamically regulated by hormonal control. In the thyroid, thyroid stimulating hormone (TSH) represses class I transcription while triggering the production of thyroglobulin and secretion of thyroid hormone which, in turn, stimulates class I gene transcription. Together, these two hormones generate a dynamic cycle of class I regulation. These findings led us to suggest that this dynamic regulation maintains a constant level of cell surface presentation of self antigens; failure to appropriately regulate MHC class I genes would lead to excessive presentation of self antigens, and contribute to the generation of autoimmune disease. Consistent with this hypothesis, we have shown that in experimental models of autoimmune systemic lupus erythematosus and blepharitis, animals that fail to express class I are resistant to disease. This resistance is not due to the failure to generate CD8+ T cells in the absence of class I, since CD8-/- animals are highly susceptible to disease. Rather, the resistance appears to be due the failure to express class I in the periphery. Thus, in adoptive transfer experiments, susceptibility to the SLE-like disease is determined by the class I status of the recipient, not the class I status of the donor spleen cells. To study the role of class I in the etiology of spontaneous autoimmune disease, we have examined the NZBxNZW murine model of SLE. We have found that in NZBxNZW mice, levels of class I expression increase significantly with age. MMI-treatment, which prevents experimental SLE, also reduces the severity and incidence of this spontaneous autoimmune disease. To directly determine the role of class I in this spontaneous disease, the 2-microglobulin knock-out gene was introduced into each of the parental strains. Development of disease was monitored in the class I deficient F2 mice.

提供针对细胞内病原体的免疫监视的MHC I类基因受到激素控制的动态调节。在甲状腺中，促甲状腺激素（TSH）抑制I类基因转录，同时触发甲状腺球蛋白的产生和甲状腺激素的分泌，这反过来又刺激I类基因转录。这两种激素共同产生I类调节的动态循环。这些发现使我们认为，这种动态调节维持了细胞表面呈递自身抗原的恒定水平;未能适当调节MHC I类基因将导致自身抗原的过度呈递，并有助于自身免疫性疾病的产生。与这一假设相一致，我们已经表明，在自身免疫性系统性红斑狼疮和睑缘炎的实验模型中，未能表达I类的动物对疾病具有抵抗力。这种抗性不是由于在缺乏I类的情况下不能产生CD 8 + T细胞，因为CD 8-/-动物对疾病高度易感。相反，阻力似乎是由于未能在外围表达I类。因此，在过继转移实验中，对狼疮样疾病的易感性取决于受体的I类状态，而不是供体脾细胞的I类状态。 为了研究I类在自发性自身免疫性疾病病因学中的作用，我们检查了SLE的NZBxNZW小鼠模型。我们已经发现，在NZBxNZW小鼠中，I类表达水平随着年龄的增长而显著增加。MMI治疗可预防实验性SLE，也可降低这种自发性自身免疫性疾病的严重程度和发病率。为了直接确定I类在这种自发性疾病中的作用，将β 2-微球蛋白敲除基因引入每个亲本菌株中。在I类缺陷型F2小鼠中监测疾病的发展。