Dynamic structural study of scaffolding proteins FtsZ and MipZ in Rhodobacter sphaeroides using ultra-high spatial-temporal resolution structured illu

利用超高时空分辨率结构化图像研究球形红杆菌中支架蛋白 FtsZ 和 MipZ 的动态结构

• 批准号: 1947281
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1947281
• 关键词: Dynamic; structural; study; scaffolding; proteins

This project focuses on developing a novel microscopy tool for live cell imaging which combines a Solid Immersion Lens with Structured Illumination Microscopy. It is thought that this technique will provide improved spatial and temporal resolution allowing greater understanding of cellular organisation, particularly in organisms as small as bacteria. The project will also employ other imaging techniques such as 3D Photo-activated Localisation Microscopy, Spinning Disk Confocal Microscopy Super Resolution Radial Fluctuations and Single Particle Tracking to compliment the primary technique being developed.This imaging approach will be tested on Rhodobacter sphaeroides, a gram negative purple bacterium which is widely studied in the Armitage Lab. The focus will be to image two key proteins employed in cell division; FtsZ and MipZ. Both form polymer rings at the divisome of the cell prior to division, however the exact behaviour and position of these rings relative to each other cannot be resolved using techniques currently available. WCUB, ENWW

本项目致力于开发一种新型的活细胞成像显微镜工具，它结合了固体浸没透镜和结构照明显微镜。人们认为，这项技术将提供更高的空间和时间分辨率，使人们能够更好地了解细胞组织，特别是在像细菌这样小的生物体中。该项目还将使用其他成像技术，如3D光激活定位显微镜、旋转圆盘共焦显微镜、超分辨率径向波动和单粒子跟踪，以补充正在开发的主要技术。该成像方法将在Armitage实验室广泛研究的革兰氏阴性紫色细菌球形红杆菌上进行测试。重点将是想象细胞分裂中使用的两种关键蛋白质：FtsZ和MipZ。两者都在分裂前在细胞的分裂体处形成聚合物环，然而这些环相对于彼此的确切行为和位置不能用目前可用的技术来解决。WCUB，ENWW