Dynamic structural study of scaffolding proteins FtsZ and MipZ in Rhodobacter sphaeroides using ultra-high spatial-temporal resolution structured illu

利用超高时空分辨率结构化图像研究球形红杆菌中支架蛋白 FtsZ 和 MipZ 的动态结构

• 批准号: 1947281
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1947281
• 关键词: Dynamic; structural; study; scaffolding; proteins

This project focuses on developing a novel microscopy tool for live cell imaging which combines a Solid Immersion Lens with Structured Illumination Microscopy. It is thought that this technique will provide improved spatial and temporal resolution allowing greater understanding of cellular organisation, particularly in organisms as small as bacteria. The project will also employ other imaging techniques such as 3D Photo-activated Localisation Microscopy, Spinning Disk Confocal Microscopy Super Resolution Radial Fluctuations and Single Particle Tracking to compliment the primary technique being developed.This imaging approach will be tested on Rhodobacter sphaeroides, a gram negative purple bacterium which is widely studied in the Armitage Lab. The focus will be to image two key proteins employed in cell division; FtsZ and MipZ. Both form polymer rings at the divisome of the cell prior to division, however the exact behaviour and position of these rings relative to each other cannot be resolved using techniques currently available. WCUB, ENWW

该项目着重于开发一种新型的显微镜工具，用于实时细胞成像，该工具将固体浸入式晶状体与结构化照明显微镜结合在一起。据认为，该技术将提供改进的空间和时间分辨率，从而使对细胞组织的了解更深入，尤其是在像细菌一样小的生物中。该项目还将采用其他成像技术，例如3D光激活的定位显微镜，旋转磁盘共聚焦显微镜超级分辨率径向波动和单个粒子跟踪来补充所开发的主要技术。将对Rhodobacter SPHAEROIDES进行测试，粒度负面紫色细菌，该方法是粒度的紫色细菌，该实验是广泛研究的。重点是成像细胞分裂中使用的两个关键蛋白。 FTSZ和MIPZ。两者都形成了分裂之前的细胞分裂组的聚合物环，但是这些环相对于彼此的确切行为和位置无法使用当前可用的技术解决。 wcub，enww