FATAL HERPESVIRUS IN ELEPHANTS

大象致命的疱疹病毒

• 批准号: 6510009
• 负责人: LAURA K RICHMAN
• 金额: $ 0.36万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6510009
• 关键词: Herpesviridae; Herpesviridae disease; Mammalia; animal mortality; death; epidemiology; genetic library; in situ hybridization; microorganism growth; polymerase chain reaction; representational difference analysis; serology /serodiagnosis; transfection; virus characteristic; virus classification; virus genetics

The purpose of this Mentored Clinical Scientist Development Award Project is to characterize, determine the source of, and prevent additional deaths from a newly recognized, fatal herpesvirus in Asian and African elephants. Reviewing cases from the last 14 years, 78 percent (7 of 9) of all young elephant deaths in North America are attributed to infection by this apparently novel systemic herpesvirus. Work to date has revealed the virus attacks small caliber vascular endothelial cells, and causes multiple hemorrhages and organ failure. Ultrastructurally, the inclusion bodies seen by light microscopy are composed of viral particles measuring 80-92 nm in diameter, morphologically consistent with the Herpesviridae family. The study design is aimed at 1) further characterization and classification of the virus utilizing polymerase chain reaction (PCR) with primers already synthesized in Dr. Hayward s laboratory, creation of a viral genomic library to obtain additional clones to help elucidate the subfamily of the virus, possible utilization of representational differential analysis, and further cultivation attempts, 2) determination of the epidemiology of the disease, including the source of the virus and mode of transmission, 3) development of a serologic test to screen potential carriers of the virus and determine rising titers in response to active infection by this herpesvirus. I (Dr. Richman) have been focusing on this disease in elephants for the past two years, working as a postdoctoral (DVM) fellow with Dr. Richard Montali at the National Zoological Park s Department of Pathology and more recently with Dr. Gary Hayward s group at the Johns Hopkins University. My plan to become an independent research scientist includes enrollment in the graduate training program in Pharmacology and Molecular Sciences in the School of Medicine at Johns Hopkins University, leading to a Ph D., in conjunction with intensive research in characterization of the elephant herpesvirus, under the direction of my mentor, Dr. Hayward.

这个指导的临床科学家发展奖的目的 项目是要表征，确定的来源并防止 亚洲新认可的致命疱疹病毒的其他死亡 和非洲大象。 审查过去14年的案件，78 北美所有年轻大象死亡的百分比（9中的7个）是 归因于这种明显新颖的系统性疱疹病毒感染。 迄今为止的工作显示了病毒攻击小口径血管 内皮细胞，并引起多个出血和器官衰竭。 超大型结构，光学显微镜看到的包含体是 由直径80-92 nm的病毒颗粒组成， 形态学上与疱疹病毒家族一致。 研究 设计的目的是1）进一步的表征和分类 利用聚合酶链反应（PCR）的病毒已经与引物 在海沃德博士的实验室中合成，创建病毒基因组 库获取其他克隆以帮助阐明 病毒，可能利用代表性差异 分析和进一步的培养尝试，2）确定 该疾病的流行病学，包括病毒和模式的来源 传输，3）开发血清学测试以筛选潜力 病毒的载体并确定响应活跃的滴度上升 这种疱疹病毒感染。 我（里奇曼博士）一直专注于 在过去的两年中，这种大象的这种疾病是 国民的理查德·蒙塔利（Richard Montali）博士（DVM）研究员（DVM）研究员 动物园病理学系，最近与博士一起 约翰霍普金斯大学的加里·海沃德（Gary Hayward）小组。 我的计划成为 独立的研究科学家包括入学 学校药理学和分子科学培训计划 约翰·霍普金斯大学（Johns Hopkins University）的医学 与大象表征的深入研究结合 疱疹病毒，在我的导师海沃德博士的指导下。