The molecular and biochemical mechanisms underlying Anthracnose resistance in Capsicum Acronym: ANTHRACAP

辣椒炭疽病抗性的分子和生化机制 缩写：ANTHRACAP

• 批准号: 1955008
• 金额: --
• 依托单位: Royal Holloway University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1955008
• 关键词: molecular; biochemical; mechanisms; underlying; Anthracnose

Background. Anthracnose (Collectrichum spp) causes pre and post-harvest fruit rot, leading to serve global economic losses. In the case of Pepper (hot and Sweet) anthracnose infections have become one of the main barriers to production. Our industrial partner, Syngenta, has identified several genotypes within collections of natural diversity that display resistance to Anthracnose. Segregating populations have been generated in which resistance to Colletotrichum species has been identified and mapped. These resulting QTL have now been fixed, enabling the study of underpinning biochemical and molecular mechanisms responsible for the Anthracnose resistance traits. Aims and Objectives. The proposed project activities aim to elucidate the biochemical and molecular mechanisms associated with Anthracnose resistance in pepper (Capsicum). To achieve this aim the following objectives will be carried out. Objective 1. The exploitation of metabolomics, proteomics and transcriptomics to ascertain the underlying molecular and biochemical components responsible for conferring resistance to Colletotrichum and potentially other fungal pathogens.Objective 2. Identify constitutive or induced antifungal metabolites synthesised by pepper genotypes that have potential broad applications as antimicrobial agents. This approach will involve natural product chemistry and bioassays.Objective 3. Functionally characterise candidate gene(s) identified in (i) using Virus Induced Gene Silencing (VIGS) in pepper (developed by previous iCASE students) or through the use of stable transformation in tomato.

背景资料。炭疽病(Collectrichum Spp)在果实收获前后引起腐烂，造成全球经济损失。就辣椒(辣和甜)而言，炭疽病已成为生产的主要障碍之一。我们的工业合作伙伴先正达已经在自然多样性的集合中确定了几种对炭疽病表现出抗性的基因类型。已经产生了分离群体，其中对炭疽菌物种的抗性已经被鉴定和定位。这些由此产生的QTL现在已经被固定，使得研究炭疽病抗性性状的生物化学和分子机制成为可能。目的和目的。拟议的项目活动旨在阐明与辣椒(Capsicum)抗炭疽病相关的生化和分子机制。为实现这一目标，将实现以下目标。目的1.利用代谢组学、蛋白质组学和转录组学来确定导致对炭疽菌和潜在的其他真菌病原体产生抗性的潜在分子和生化成分。目的2.鉴定由辣椒基因型合成的具有潜在抗菌剂应用前景的结构性或诱导性抗真菌代谢物。这一方法将涉及天然产物化学和生物分析。目的3.利用病毒诱导的基因沉默(VIGS)在辣椒中鉴定候选基因(S)或通过番茄稳定转化获得该候选基因。