ISOLATION AND DISTR OF HIGH QUALITY HUMAN PANCRE*

高质量人胰腺的分离和分配*

• 批准号: 6530153
• 负责人: Ali Naji
• 金额: $ 64.01万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530153
• 关键词: calcium flux; cooperative study; cyclic AMP; diabetes mellitus; diabetes mellitus therapy; glucose metabolism; hormone regulation /control mechanism; human subject; human therapy evaluation; insulin; nonhuman therapy evaluation; pancreatic islet transplantation; pancreatic islets; patient oriented research; postmortem; pulmonary respiration

DESCRIPTION (Provided by applicant): The transplant program at the University of Pennsylvania (UP)has had a long-standing interest in the application of pancreatic islet transplantation in the treatment of diabetes mellitus. The program has continued basic investigations of the biological barriers to islet transplantation, with emphasis on the induction of immunological tolerance. Insights gained from experimental islet transplantation have led to recent successful applications of this therapy and achievement of euglycemia in patients with Type I diabetes mellitus. Based on these advances, a comprehensive islet transplantation program was established at the UP integrating a multidisciplinary team of investigative scientists and clinicians with recognized expertise in diabetes research and transplantation. Since the inception of the program in 1999, significant progress has been made in building its critical components: 1) construction of an Food and Drug Administrative (FDA)-compliant current good manufacturing practices (cGMP) facility devoted exclusively to islet processing; 2) recruitment and training of personnel qualified to perform islet isolation and assignment of dedicated transplant surgeons for on-site recovery of cadaveric pancreata; 3) establishment of a strong collaboration with the local organ procurement organization, which has led to a marked increase in the procurement of the human cadaveric pancreata; 4) distribution of isolated pancreatic islets to regional and national investigators engaged in diabetes research; and 5) development of a standardized islet quality index. In the present application, this strong infrastructure will serve as the mechanism for increasing the availability of human cadaveric pancreata suitable for preparation of isolated islets. This goal will be accomplished by procurement of pancreata from an expanded donor pool, including non-heart-beating organ donors. Another emphasis for the program will be the refinement of the islet quality index, based on physiological, biochemical, and morphological parameters, for correlation with the outcome of islet transplantation. With this strong platform in place, the program is poised to realize its goal of generating large quantities of highly purified and quality-controlled human islets for transplantation into Type I insulin-dependent diabetic patients.

描述（由申请人提供）：大学的移植计划 宾夕法尼亚大学（UP）一直对应用 胰岛移植治疗糖尿病的 该计划继续对胰岛的生物屏障进行基本调查， 移植，重点是诱导免疫耐受。 从实验性胰岛移植中获得的见解导致了最近的 该疗法的成功应用和Eurobenzin的实现 1型糖尿病患者。基于这些进步，A 在北方邦建立了一个全面的胰岛移植计划 整合多学科的调查科学家团队， 在糖尿病研究和移植方面具有公认专业知识的临床医生。 自1999年该计划启动以来，已取得重大进展 在建设其关键组成部分：1）建设一个食品和药品 符合管理（FDA）的现行药品生产质量管理规范（cGMP） 专门用于胰岛加工的设施; 2）招募和培训 有资格进行胰岛分离的人员， 移植外科医生现场恢复尸体胰腺; 3） 与当地器官采购部门建立强有力的合作关系 这导致了采购量的显著增加， 人尸体胰腺; 4）分离的胰岛分布， 从事糖尿病研究的区域和国家研究人员;以及 5)制定标准化的胰岛质量指数。 在本申请中，这种强大的基础设施将充当 提高人尸体胰腺可用性的机制 适合于制备分离的胰岛。这一目标将得以实现 通过从扩大的供体库中获取胰腺，包括无心跳的 器官捐献者该计划的另一个重点将是细化 胰岛质量指数，基于生理，生化， 形态学参数，用于与胰岛结果的相关性 移植有了这个强大的平台，该计划准备 实现了生产大量高纯度， 用于移植到I型胰岛素依赖型糖尿病中的质量控制的人胰岛 糖尿病患者