Circadian regulation of gene expression and its application in synthetic biology

许多生物体具有生物钟计时机制，使它们能够适应昼夜循环。最简单的生物钟存在于光合作用的蓝细菌中--一大群在工业和生态上具有重要意义的微生物。我们将研究昼夜节律程序如何调节蓝藻中的基因表达。我们的主要目标将是确定在转录水平上打开/关闭基因的因素。理解这些基本原理对于在合成生物学中实现生物钟至关重要。最终，我们的目标是设计生物节律控制的元素，用于工业相关微生物的合成生物学应用。该项目是开发Portabolomics的大型财团的一部分，http：//www.ncl.ac.uk/computing/research/project/5355，合成生物学的创新方法，旨在提高新工业应用设计的效率和灵活性。该项目将设在纽卡斯尔大学细菌细胞生物学中心（网址：//www.ncl.ac.uk/cbc/），该中心容纳了细菌细胞生物学领域的世界领先科学家，并拥有最先进的设备。成功的候选人将获得广泛的生物化学，分子生物学和微生物学技术的培训。

Many living organisms possess circadian clock - timing mechanism allowing them to adjust to day/night cycles. The simplest version of a clock is found in photosynthetic cyanobacteria - large group of industrially and ecologically important microorganisms. We are going to investigate how circadian programme regulates gene expression in cyanobacteria. Our main goal will be to identify factors, which switch genes on/off at the level of transcription. Understanding of these fundamental principles is vital for implementation of circadian clock in synthetic biology. Ultimately, we aim to design elements of circadian control for synthetic biology applications in industrially relevant microorganisms.This project is part of large consortium developing Portabolomics, http://www.ncl.ac.uk/computing/research/project/5355,the innovative approach to synthetic biology, aiming at increasing an efficiency and flexibility of design of a new industrial applications.The project will be based in Centre for Bacterial Cell Biology of Newcastle University (http://www.ncl.ac.uk/cbcb/), which accommodates world-leading scientists in the field of biology of bacterial cell and possesses the state of the art equipment. The successful candidate will acquire training in a wide range of biochemistry, molecular biology and microbiology techniques.