DPP-mediated signaling in Drosophila embryos

果蝇胚胎中 DPP 介导的信号传导

• 批准号: 6520340
• 负责人: Anthea Letsou
• 金额: $ 23.1万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6520340
• 关键词: Drosophilidae; biological signal transduction; cell differentiation; developmental genetics; fluorescence microscopy; gel electrophoresis; gel mobility shift assay; gene expression; gene induction /repression; genetic regulation; invertebrate embryology; molecular cloning; nucleic acid hybridization; nucleic acid sequence; phosphorylation; polymerase chain reaction; western blottings

DESCRIPTION (provided by applicant): In Drosophila and similar model genetic organisms, genes playing pivotal developmental roles in embryogenesis have been identified in screens for zygotic and maternal-effect lethals. Molecular characterization of these mutated loci has been the focus of many labs' research programs over the last two decades, and consequently, the signaling events that direct patterning (e.g. DV axis determination) and morphogenetic (e.g. dorsal closure) processes have been very well characterized. Despite mutagenesis experiments that are nearly saturating, only a very few genes have been identified that fine tune the signaling process (e.g. corkscrew) and/or control the mechanics of development (e.g. concertina and folded gastrulation). Put another way, although signal transducers and transcription factors have been identified in abundance, the fine-tuners (here referred to as facilitators) and the downstream effectors of these signaling cascades have remained, in large part, unidentified and uncharacterized. The additional genes that are essential for embryonic development in Drosophila might be overlooked in genetic screens because they mutate to phenotypes affecting only one part of a complex pattern or because they do not mutate to lethality. We have exploited enhancer/suppressor and reverse genetic methods to identify Dpp signaling molecules that were missed in more traditional genetic surveys. The specific aims of the current research application are to: (1) characterize the function of eop (enhancer of punt), a newly identified component of the DPP-mediated signaling pathway, (2) characterize the role of the spatially-restricted scylla and charybde transcripts in executing DPP-mediated signaling and dorsal differentiation during embryogenesis, and (3) conduct a high-throughput screen for additional spatially-restricted transcripts and putative effectors of DPP-mediated signal transduction. We expect that the proposed studies to provide significant insight into cellular processes regulated by Dpp-mediated signal transduction.

描述（由申请人提供）：在果蝇和类似模型遗传中 生物，在胚胎发生中起关键发育作用的基因一直是 在筛选中鉴定出伴有和母体效应致死。分子 这些突变基因座的表征一直是许多实验室的重点 在过去的二十年中，研究计划，因此是信号 直接构图的事件（例如DV轴确定）和形态发生 （例如背闭合）过程的表征非常好。尽管 诱变实验几乎饱和，只有很少的基因具有 已确定对信号过程（例如开瓶器）和/或 控制开发力学（例如六角琴和折叠式胃肠道）。 换句话说，尽管信号传感器和转录因子具有 被识别为微调（这里称为 促进者）和这些信号级联的下游效应子具有 在很大程度上，仍然是未知和未表征的。附加基因 这对于果蝇中的胚胎开发至关重要 在遗传筛选中，因为它们突变为仅影响一部分的表型 复杂的模式或因为它们不突变为致死性。我们已经利用了 增强剂/抑制器和反向遗传方法识别DPP信号 在更传统的遗传调查中遗漏的分子。 当前研究应用程序的具体目的是：（1）表征 EOP（Punt的增强子）的功能，该功能是该的新确定的组件 DPP介导的信号通路，（2）表征了 执行DPP介导的空间限制性Scylla和Charybde成绩单 胚胎发生过程中的信号传导和背分化，（3）进行A 高通量屏幕，用于其他空间限制的成绩单和 DPP介导的信号转导的推定效应子。我们期望 拟议的研究提供了对细胞过程的重大见解 由DPP介导的信号转导调节。