U Guam RISE equipment supplement for R25 GM63682-01

描述(由申请人提供)：这里要求的三种仪器将显著改善关岛大学学生和教职员工的生物医学研究环境。这将立即服务于学生和教师发展的日益增长的目标，并将促进教学和研究的一体化，这将激励更多的学生完成学士学位并继续深造，以及更多的教师发表和申请研究补助金。所需的三台仪器包括一台低场核磁共振(核磁共振)光谱仪、一台VersaDoc 1000成像仪和一台差分干涉对比(DIC)/荧光显微镜，以支持对学生进行现代显微镜培训。每个仪器将服务于我们所有的生物和化学专业(-60-70名学生)，通过9门化学和8门生物课程。通过在不同的课程中使用这些工具，学生将发展在研究中将这些技术应用于新情况的能力。这些设备还将用于教师的研究项目，通常是与学生一起进行的，这将增加演示文稿和出版物的产出，并为拨款申请提供初步数据。来自成像仪和显微镜的数据将通过新的Rise Science局域网共享。已拨出经费，由指定教员负责大学的设备维护工作。核磁共振是有机和生物化学中最重要和最强大的光谱技术之一，但目前在关岛还没有。最适合我们教学需要、机构规模小、位置偏远的核磁共振是一台翻新的60 MHz傅里叶变换光谱仪，配备了永久磁铁和1H和_C探头。该系统比超导300 MHz仪器更具弹性，不需要低温，并且可以由大学利用现有资源进行维护。负责人化学家去年参加了NSF赞助的核磁共振研讨会，VersaDoc成像仪和相关的统计分析将使我们能够将遗传学和分子实验室推进到定量分析。学生将学习设计精确的实验以准确估计种群遗传参数，并将能够实施他们在生物医学研究中所需的自动化、定量测量类型。使用DIC/FL显微镜，学生将学习选择和设置合适的光学元件用于生物多样性和细胞学研究。来自DIC光学显微镜的活生物标本的优越视角将加深学生对生物系统的理解，并进一步促进他们对关岛海洋生物群研究的贡献。

DESCRIPTION (provided by applicant): The three instruments requested here will significantly enhance the biomedical research climate for students and faculty at the University of Guam. This will immediately serve the RISE goals of student and faculty development, and will add to integration of teaching and research that will motivate more students to complete the baccalaureate degree and go on to graduate school, and more teaching faculty to publish and apply for research grants. The three instruments requested include a low-field nuclear magnetic resonance (NMR) spectrometer, a VersaDoc 1000 Imager, and a differential interference contrast (DIC)/fluorescence microscope to support training of students in modern microscopy. Each of these instruments will serve all our biology and chemistry majors (-60-70 students) through 9 chemistry and 8 biology courses. By using the instruments in different courses, students will develop the ability to apply the techniques to novel situations in their research. The equipment will also be used in faculty research projects, often with students, which will increase output of presentations and publications and contribute preliminary data toward grant applications. The Data from the Imager and microscope will be shared through the new RISE Science LAN. Provision has been made for maintenance of the equipment by the University under the responsibility of the named faculty. NMR is one of the most important and powerful spectroscopic techniques in organic and biochemistry, but is not currently available on Guam. The NMR most suited to our teaching needs, small institution size, and remote location is a refurbished 60 MHz Fourier Transform spectrometer equipped with a permanent magnet and 1H and _C Probes. This system is much more resilient than a superconducting 300 MHz instrument, requires no cryogenics, and can and maintained by the University with available resources. The chemist in charge attended an NSF-sponsored workshop on NMR last year The VersaDoc Imager and associated statistical analyses will allow us to advance the genetics and molecular labs into quantitative analysis. Students will learn to design experiments with precision to accurately estimate population genetic parameters and will be able to implement the types of automated, quantitative measurements they will need in biomedical research. With the DIC/FL microscope students will learn to select and set up appropriate optics for biodiversity and cytology studies. The superior views of living biological specimens from DIC optics in the microscope will deepen students' understanding of biological systems and further their contribution to research into the marine biota of Guam.