MAJOR HISTOCOMPATIBILITY MOLECULES IN LUTEAL FUNCTION

描述：(改编自申请者的摘要)本研究旨在 对于卵巢生物学中的一个重要问题，潜在的机制是通过 黄体在每个发情周期后被处理掉。私家侦探已经 对奶牛类固醇生成黄体细胞进行了重要观察 表达II类主要组织相容性分子，这是一种常见的特性 仅限于专业抗原呈递免疫/炎症细胞。这是不同寻常的 黄体细胞的特性和其他观察结果导致P.I. 有趣而具有挑衅性的假设，黄体细胞是 短暂性自身免疫反应的参与者，这会导致排斥反应 每个周期结束时的黄体组织。因此，我们的目标是：1)描述 与抗原呈递相关的必需元件的表达 I类和II类主要组织相容性分子；2)研究 调节组成抗原的特定蛋白的表达 3)研究其表达、功能及意义。 共刺激分子的调节；4)决定 黄体细胞和T淋巴细胞通过MHC-I和/或MHC介导 II类-受限交互；5)确定 MHC第I类和第II类分子表达的多肽 功能性与退化性黄体。这些研究预计将揭示 黄体细胞是否具有抗原提呈的必要特征 单元格，从而提供新信息作为对提议的瞬变的测试 黄体退化过程中的自身免疫反应。

DESCRIPTION: (Adapted from the applicant's abstract) This research is directed to an important problem in ovarian biology, the underlying mechanisms through which the corpus luteum is disposed of after each estrous cycle. The P. I. has made the important observation that the steroidogenic luteal cells of the cow express class II major histocompatibility molecules, a property normally found only on professional antigen presenting immune/inflammatory cells. This unusual property of luteal cells and other observations have led the P. I. to the interesting and provocative hypothesis, that the luteal cells are the participants in a transient autoimmune response, which leads to rejection of the luteal tissue at the end of each cycle. Thus the Aims are: 1) characterize the expression of elements necessary for antigen presentation associated with class I and class II major histocompatibility molecules; 2) investigate the regulation of expression of specific proteins that comprise the antigen processing complex; 3) investigate the expression, functional significance and regulation of co-stimulatory molecules; 4) determine whether engagement between luteal cells and T lymphocytes is mediated through MHC class I and /or MHC class II-restricted interaction; 5) determine if alterations occur in the peptides presented by MHC class I and class II molecules expressed by functional vs. regressing corpora lutea. These studies are expected to reveal whether luteal cells have the requisite characteristics of antigen presenting cells, thus providing new information as test of a proposed transient autoimmune reaction during regression of the corpus luteum.