MAJOR HISTOCOMPATIBILITY MOLECULES IN LUTEAL FUNCTION

描述：（改编自申请人的摘要）本研究旨在 卵巢生物学中的一个重要问题， 黄体在每个发情周期后被处理掉。私家侦探。具有 做了重要的观察，牛的类固醇黄体细胞 表达II类主要组织相容性分子，这是一种通常发现的性质， 仅对专职抗原呈递免疫/炎症细胞。这种不寻常 黄体细胞的性质和其他观察导致了P.I.到 一个有趣的和挑衅性的假设，即黄体细胞是 参与短暂的自身免疫反应，导致排斥反应， 在每个周期结束时的黄体组织。因此，目标是：1）表征 抗原呈递所必需的元件的表达与 I类和II类主要组织相容性分子; 2）研究 调节包含抗原的特定蛋白质的表达 3）探讨了加工复合体的表达、功能意义和 共刺激分子的调节; 4）确定 黄体细胞和T淋巴细胞通过MHC I类和/或MHC II类限制性相互作用; 5）确定是否发生改变， 由MHC I类和II类分子呈递的肽， 功能性与退化性黄体。这些研究有望揭示 黄体细胞是否具有抗原呈递的必要特征 细胞，从而提供新的信息作为测试的建议瞬态 黄体退化期间的自身免疫反应。

DESCRIPTION: (Adapted from the applicant's abstract) This research is directed to an important problem in ovarian biology, the underlying mechanisms through which the corpus luteum is disposed of after each estrous cycle. The P. I. has made the important observation that the steroidogenic luteal cells of the cow express class II major histocompatibility molecules, a property normally found only on professional antigen presenting immune/inflammatory cells. This unusual property of luteal cells and other observations have led the P. I. to the interesting and provocative hypothesis, that the luteal cells are the participants in a transient autoimmune response, which leads to rejection of the luteal tissue at the end of each cycle. Thus the Aims are: 1) characterize the expression of elements necessary for antigen presentation associated with class I and class II major histocompatibility molecules; 2) investigate the regulation of expression of specific proteins that comprise the antigen processing complex; 3) investigate the expression, functional significance and regulation of co-stimulatory molecules; 4) determine whether engagement between luteal cells and T lymphocytes is mediated through MHC class I and /or MHC class II-restricted interaction; 5) determine if alterations occur in the peptides presented by MHC class I and class II molecules expressed by functional vs. regressing corpora lutea. These studies are expected to reveal whether luteal cells have the requisite characteristics of antigen presenting cells, thus providing new information as test of a proposed transient autoimmune reaction during regression of the corpus luteum.