CORE--NMR

核磁共振核心设施提供该中心和其他单位， 最先进的核磁共振光谱仪核磁共振设备，目前 位于化学系的实验室是以“开放式”的方式运作的。 博士迈克尔·斯通是核心的领导者，他由马库斯先生协助 Voehler负责操作，维护和维护设备，并培训新的 用户. Stan Bottoms先生是一位电子专家， 也被利用。中心的调查人员，哈里斯博士谁有一个有影响力的 在设施运营中的角色为中心提供咨询 人员的他和马内特博士是斯通博士的主要合作者。 常规分析以及先进的多维核磁共振技术， 进行复杂的结构。目前有200MHz，300MHz，400MHz 500MHz的仪器。500MHz NMR已升级 包括3RF通道和场梯度功能，以及控制台升级 300 MHz和400 MHz仪器的测试正在进行中。共6个硅 图形工作站用于处理光谱和进行分子 建模和动力学研究。新的伙伴关系已经建立， 范德比尔特大学医学中心，范德比尔特大学中心， 包括艺术与科学学院、皮博迪学院和 工程部将操作一个新指定的范德比尔特大学磁 谐振中心（VUMRC）。除了现有的设备，600MHz 800兆赫核磁共振仪和3特斯拉全身成像系统将 添加到这个中心。额外的技术支持，以协助目前的 核磁共振专家Markus Voehler将被任命。预计各国 博士斯通和沃勒将参与新的 大学范围内的VUMRC。在本财政年度内，中心 研究人员构成了核磁共振核心的大部分利用 设施。到目前为止，该设施的最大用户是DNA成员 损伤和突变研究核心。然而，该设施还提供服务， 酶的氧化与结合研究的核心--氧化损伤 研究核心，和环境病理学研究核心。九十二 同行评审的出版物报道了从NMR设施获得的结果。 有一个计划，优先使用核磁共振核心设施。有 两种类型的用户;那些需要常规核磁共振服务的结构 证明，以及那些使用全方位的2-D功能的结构 生物工作。前者的工作主要是用300 MHz NMR完成的， 而其他三种仪器专用于二维和其他先进的 技术在预定的基础上。600和800兆赫的仪器，当他们 arrive也将用于后一目的。获得文书 在核心设施中，通过Markus Voehler的"动手"培训或通过 与中心研究者或核心人员合作。在下一次拨款中 预计环境部将更多地使用核磁共振设施。 病理学和酶的氧化和共轭研究核心。中心 支付中心人员每小时使用费的75%，减去 由其学术单位提供。该中心还提供了一部分（15%） 斯通博士的吗的薪水。该中心还为促进 购买新设备。总的来说，中心对实验室的贡献 营业费用直接与其利用率成比例， 设备.

The NMR Core Facility provides the Center and other units on campus with state-of-the-art NMR spectroscopy. The NMR facility, presently located in the Chemistry Department is operated on an "open shop" basis. Dr. Michael Stone is the leader of the Core and he is assisted by Mr. Markus Voehler who operates, troubleshoots and maintains the equipment and trains new users. The services of Mr. Stan Bottoms, a staff electronics specialist are also utilized. Center investigators, Drs. Harris who has had an influential role in the operation of the facility provides consultation to Center personnel. He and Dr. Marnett are major collaborators with Dr. Stone. Routine analyses as well as advanced multi-dimensional NMR techniques for complex structures are performed. There are presently 200MHz, 300MHz, 400MHz and 500MHz instruments within the facility. The 500MHz NMR has been upgraded to include 3RF channels and field gradient capabilities, and console upgrades are underway for the 300MHz and 400MHz instruments. A total of six Silicon Graphics workstations are used to work up spectra and conduct molecular modeling and dynamics studies. A new partnership has been created between the Vanderbilt University Medical Center, Vanderbilt University Central which includes the College of Arts and Science, the Peabody College and the School of Engineering to operate a newly designated Vanderbilt University Magnetic Resonance Center (VUMRC). In addition to the existing equipment, a 600MHz and an 800MHz NMR as well as a 3 Tesla whole body imaging system will be added to this Center. Additional technical support to assist the current NMR specialist, Markus Voehler will be appointed. It is anticipated that Dr. Stone and Mr. Voehler will be involved in the management of the new University-wide VUMRC. During the present funding period, Center investigators constituted the majority of utilization of the NMR Core Facility. By far the heaviest users of the facility were members of the DNA Damage and Mutagenesis Research Core. The facility, however, also serviced the Enzymatic Oxidation and Conjugation Research Core, the Oxidative Damage Research Core, and the Environmental Pathology Research Core. Ninety-two peer-reviewed publications reported results obtained from the NMR facility. A plan exists for prioritizing the usage of the NMR Core Facility. There are two types of users; those who require routine NMR services for structure proof, and those who use the full range of 2-D capabilities for structural biology work. The work for the former is done largely with the 300 MHZ NMR, while the other three instruments are dedicated for 2-D and other advanced techniques on a scheduled basis. The 600 and 800 MHZ instruments when they arrive will also be used for the latter purposes. Access to the instruments in the Core Facility are through "hands on" training by Markus Voehler or via collaboration with Center investigators or Core personnel. In the next grant period heavier use of the NMR Facility is expected from the Environmental Pathology and Enzymatic Oxidation and Conjugation Research Cores. The Center contributes 75% of the hourly use charges accrued by Center personnel, less is contributed by their academic units. The Center also provides a portion (15%) of Dr. Stone?s salary. The Center has also contributed considerably to the purchase of new equipment. Overall, the Center contribution to the laboratory operating expenses is directly in proportion of its utilization of the equipment.