CORE--IMMUNOLOGY

核心--免疫学

• 批准号: 6564372
• 负责人: Jerry R McGhee
• 金额: $ 16.54万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564372
• 关键词: T lymphocyte; biomedical facility; cytotoxic T lymphocyte; genetically modified animals; helper T lymphocyte; immunology; inflammation; laboratory mouse; leukocyte activation /transformation; mucosal immunity; transfection /expression vector

Description: (Taken directly from the application) In order to obtain a better understanding of the induced mucosal and systemic immune responses to both transgene and vector, the associated T cell subset responses which include CD4+, CD8+, alpha beta and gamma-delta T cells and mucosal antibody responses in the respiratory tract and systemic compartment will be analyzed in the Immunology Core. The activation of CD4+ T helper type 1 (Th1) cells for the induction of cell-mediated immunity (as manifested by delayed type hypersensitivity) which contribute to inflammation and antibody mediated immunity regulated by Th2-type helper cells will be analyzed in the respiratory tract and associated lymph nodes. The roles for key Th1 and Th2 cytokines like IFN-y and IL-4 for the induction of these two T helper cell subsets will be assessed by the use of cytokine gene disrupted (knockout) mice. This will contribute to our understanding of vector-specific immunity and may reveal pathways to specifically decrease these responses by the absence or over-expression of specific cytokines. In addition to Th1 and Th2 cells, it is also clear that gamma delta T cells can play an incompletely understood role in the mucosal immune response. Therefore, our studies in the Core will address their potential role in mucosal and systemic immunity to vector and transgene by assessing their contribution in normal and gamma delta TCR-deficient mice. This Immunology Core will provide state-of-the-art analyses of Th1- and Th2-type and CTL responses to various constructs developed by CF gene Therapy Core Center investigators and pilot projects. In so doing, we will help optimize the vector and transgene for effective therapy with minimum inflammation.

描述：（直接取自应用程序） 为了更好地了解诱导的粘膜和全身 对转基因和载体的免疫应答，相关的T细胞亚群 包括CD 4+、CD 8+、α β和γ-δ T细胞的应答， 呼吸道和全身隔室中的粘膜抗体应答 将在免疫学核心中进行分析。CD 4 + T辅助细胞1型的激活 (Th1)用于诱导细胞介导的免疫的细胞（如通过 迟发型超敏反应），其有助于炎症和抗体 由Th 2型辅助细胞调节的介导免疫将在 呼吸道和相关淋巴结。关键Th 1和Th 2的作用 细胞因子如IFN-γ和IL-4用于诱导这两种T辅助细胞 通过使用细胞因子基因破坏（敲除）小鼠来评估亚群。 这将有助于我们理解媒介特异性免疫， 揭示途径，以具体减少这些反应的缺乏或 特异性细胞因子的过度表达。除了Th 1和Th 2细胞外， 同样清楚的是，γ δ T细胞可以发挥不完全理解的作用， 粘膜免疫反应。因此，我们在核心的研究将解决 它们在对载体和转基因的粘膜和全身免疫中的潜在作用 通过评估它们在正常和γ δ TCR缺陷小鼠中的作用。 该免疫学核心将提供最先进的Th 1和 CF基因疗法开发的各种构建体的Th 2型和CTL应答 核心中心调查员和试点项目。这样做，我们将帮助 优化载体和转基因， 炎症