HUMAN B CELL TRANSFORMATION BY EPSTEIN BARR VIRUS

爱泼斯坦巴尔病毒对人类 B 细胞的转化

• 批准号: 6510013
• 负责人: JENNIFER M MARTIN
• 金额: $ 9.03万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6510013
• 关键词: B lymphocyte; Epstein Barr virus; biological signal transduction; cell transformation; intermolecular interaction; mutant; protein structure function; virus protein

In this project, the investigator proposes to analyze the contribution of latent membrane protein 1 (LMP-1) of EBV to immortalization of cells. She will use molecular genetic and biochemical analyses to determine the transmembrane topology of LMP-1, to study the quaternary structure of LMP-1 and its relevance to function, and determine the relationship between the function of the amino -terminus of LMP-1 and its structure, cytoskeletal association, turnover, and membrane patching. The specific aims of the research are (1) to determine the transmembrane topology of the LMP-1 protein in the immortalized cell, (2) to determine if homo- oligomerization is required for function of the LMP-1 in immortalization, and (3) to determine how the structure of the cytoplasmic N-terminus of LMP-1 relates to function and the biochemical properties of cytoskeletal association, membrane patching, and rapid turnover, all of which suggest receptor-ligand function. The investigator points out that while it is clear that the N-terminal of LMP-1 is responsible for transformation by EBV, there is controversy as to whether this is due simply to the structure of the molecule in the membrane, or due to protein-protein interactions. Eventually, the investigator hopes to determine how LMP-1 functions in signal transduction leading to immortalization of B cells.

在这个项目中，研究者建议分析 EB病毒潜伏膜蛋白1（LMP-1）与细胞永生化的关系。 她将使用分子遗传学和生物化学分析来确定 LMP-1的跨膜拓扑结构，以研究LMP-1的四级结构。 LMP-1与其相关功能，并确定关系 LMP-1的氨基末端的功能与其结构之间， 细胞骨架结合、周转和膜修补。 具体 本研究的目的是（1）确定跨膜拓扑结构， 永生化细胞中的LMP-1蛋白，（2）确定是否同源- 低聚是LMP-1在细胞中发挥功能所必需的。 （3）确定结构如何， LMP-1的胞质N-末端与功能和生化特性有关， 细胞骨架的性质，膜修补，和快速 所有这些都表明受体-配体功能。 的 研究人员指出，虽然很明显， LMP-1负责EBV的转化，存在争议， 这是否仅仅是由于分子的结构， 膜，或由于蛋白质-蛋白质相互作用。 最终 研究人员希望确定LMP-1在信号传导中的功能 转导导致B细胞的永生化。