Median Preoptic Nucleus and the Control of Sleep

正中视前核和睡眠的控制

• 批准号: 6430418
• 负责人: Ronald Szymusiak
• 金额: $ 22.61万
• 依托单位: SEPULVEDA RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6430418
• 关键词: angiotensin II; blood osmolarity; body temperature regulation; brain electrical activity; cell population study; computer data analysis; electroencephalography; electromyography; fos protein; gamma aminobutyrate; gene expression; glutamate decarboxylase; immunocytochemistry; laboratory rat; microelectrodes; neuroanatomy; neurochemistry; neurogenetics; neurons; neuroregulation; polygraphy; preoptic areas; psychic activity level; saline; sleep regulatory center

A role for the preoptic area (POA) of the hypothalamus in the control of sleep is supported by the results of lesion, stimulation and neuronal recording experiments. The precise location of putative sleep-regulatory cell groups within the POA is incompletely understood, but recent progress has been made through application of the c-fos protein immunoreactivity (IR) method. A localized group of neurons in the ventrolateral POA (vlPOA) that exhibit Fos-IR during sleep has been described in rat. vlPOA neurons have been shown to project to monoaminergic arousal systems in the posterior hypothalamus and brainstem and to contain the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Neuronal recording studies confirmed the presence of neurons with sleep-related discharge patterns within the vlPOA. The goal of proposed work is to characterize the anatomy and physiology of a second POA region where neurons exhibiting seep- related Fos-IR have been localized, the median preoptic nucleus (MnPN). Proposed experiments will answer the following questions: 1) Does the MnPN contain a high concentration of neurons with sleep-related discharge patterns, like that previously found in the vlPOA? 2) Are MnPN neurons that exhibit sleep-related Fos-IR also GABAergic? 3) Do MnPN neurons that exhibit sleep-related Fos-IR project to arousal systems in the magnocellular basal forebrain, the perifornical region of the lateral hypothalamus, the dorsal raphe nucleus and the dorsolateral pons? 4) Are a subset of MnPN neurons with sleep- related discharge also thermosensitive, as has been previously shown for vlPOA neurons? 5) Are MnPN neurons that are involved in the control of body fluid homeostasis a separate population from sleep-related MnPN neurons? Proposed work will examine in depth the anatomical, neurochemical, and electrophysiological features of an important POA neuronal group, the MnPN. Planned experiments will contribute to a more complete understanding of the role of hypothalamic mechanisms in normal and disordered sleep and arousal states.

下丘脑视前区（POA）在控制睡眠中的作用得到了损伤、刺激和神经元记录实验的支持。睡眠调节细胞群在POA中的确切位置尚不完全清楚，但最近通过应用c-fos蛋白免疫反应性（IR）方法取得了进展。在大鼠的睡眠中，腹外侧POA （vlPOA）中有一组局部神经元表现出Fos-IR。vlPOA神经元已被证明投射到下丘脑后部和脑干的单胺能唤醒系统，并含有抑制性神经递质γ -氨基丁酸（GABA）。神经元记录研究证实了在vlPOA内存在与睡眠相关的放电模式的神经元。本研究的目标是描述第二个POA区域的解剖和生理学特征，其中神经元表现出与渗漏相关的Fos-IR，即正中视前核（MnPN）。提出的实验将回答以下问题：1)MnPN是否含有高浓度的与睡眠相关的放电模式的神经元，就像之前在vlPOA中发现的那样？2)表现出睡眠相关Fos-IR的MnPN神经元是否也能产生gaba ？3)表现出与睡眠相关的Fos-IR的MnPN神经元是否对基底大细胞前脑、下丘脑外侧皮层周围区、中音背核和脑桥背外侧的觉醒系统有影响？4)与睡眠相关放电的MnPN神经元子集是否也像先前在vlPOA神经元中所显示的那样具有热敏性？5)参与体液稳态控制的MnPN神经元是否与睡眠相关的MnPN神经元分开？拟议的工作将深入研究重要的POA神经元群MnPN的解剖、神经化学和电生理特征。计划中的实验将有助于更全面地了解下丘脑机制在正常和紊乱睡眠和觉醒状态中的作用。