Median Preoptic Nucleus and the Control of Sleep

正中视前核和睡眠的控制

• 批准号: 7036540
• 负责人: Ronald Szymusiak
• 金额: $ 14.42万
• 依托单位: SEPULVEDA RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7036540
• 关键词: angiotensin II; blood osmolarity; body temperature regulation; brain electrical activity; cell population study; computer data analysis; electroencephalography; electromyography; fos protein; gamma aminobutyrate; gene expression; glutamate decarboxylase; immunocytochemistry; laboratory rat; microelectrodes; neuroanatomy; neurochemistry; neurogenetics; neurons; neuroregulation; polygraphy; preoptic areas; psychic activity level; saline; sleep regulatory center

A role for the preoptic area (POA) of the hypothalamus in the control of sleep is supported by the results of lesion, stimulation and neuronal recording experiments. The precise location of putative sleep-regulatory cell groups within the POA is incompletely understood, but recent progress has been made through application of the c-fos protein immunoreactivity (IR) method. A localized group of neurons in the ventrolateral POA (vlPOA) that exhibit Fos-IR during sleep has been described in rat. vlPOA neurons have been shown to project to monoaminergic arousal systems in the posterior hypothalamus and brainstem and to contain the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Neuronal recording studies confirmed the presence of neurons with sleep-related discharge patterns within the vlPOA. The goal of proposed work is to characterize the anatomy and physiology of a second POA region where neurons exhibiting seep- related Fos-IR have been localized, the median preoptic nucleus (MnPN). Proposed experiments will answer the following questions: 1) Does the MnPN contain a high concentration of neurons with sleep-related discharge patterns, like that previously found in the vlPOA? 2) Are MnPN neurons that exhibit sleep-related Fos-IR also GABAergic? 3) Do MnPN neurons that exhibit sleep-related Fos-IR project to arousal systems in the magnocellular basal forebrain, the perifornical region of the lateral hypothalamus, the dorsal raphe nucleus and the dorsolateral pons? 4) Are a subset of MnPN neurons with sleep- related discharge also thermosensitive, as has been previously shown for vlPOA neurons? 5) Are MnPN neurons that are involved in the control of body fluid homeostasis a separate population from sleep-related MnPN neurons? Proposed work will examine in depth the anatomical, neurochemical, and electrophysiological features of an important POA neuronal group, the MnPN. Planned experiments will contribute to a more complete understanding of the role of hypothalamic mechanisms in normal and disordered sleep and arousal states.

损伤、刺激和神经元记录实验的结果支持了下丘脑视前区（POA）在控制睡眠中的作用。 POA 内假定的睡眠调节细胞群的精确位置尚不完全清楚，但最近通过 c-fos 蛋白免疫反应 (IR) 方法的应用取得了进展。 大鼠腹外侧 POA (vlPOA) 中的一组局部神经元在睡眠期间表现出 Fos-IR。 vlPOA 神经元已被证明投射到下丘脑后部和脑干的单胺能唤醒系统，并含有抑制性神经递质 γ-氨基丁酸 (GABA)。 神经元记录研究证实了 vlPOA 内存在与睡眠相关的放电模式的神经元。拟议工作的目标是表征第二个 POA 区域的解剖学和生理学特征，其中表现出渗出相关 Fos-IR 的神经元已定位，即正中视前核 (MnPN)。 拟议的实验将回答以下问题：1）MnPN 是否包含高浓度的具有睡眠相关放电模式的神经元，就像之前在 vlPOA 中发现的那样？ 2) 表现出与睡眠相关的 Fos-IR 的 MnPN 神经元是否也是 GABA 能的？ 3）表现出与睡眠相关的Fos-IR的MnPN神经元是否投射到大细胞基底前脑、外侧下丘脑的穹窿周区域、中缝背核和脑桥背外侧的唤醒系统？ 4) 具有睡眠相关放电的 MnPN 神经元子集是否也具有热敏感性，正如之前针对 vlPOA 神经元所显示的那样？ 5) 参与体液稳态控制的 MnPN 神经元是否与睡眠相关的 MnPN 神经元不同？拟议的工作将深入研究重要的 POA 神经元群 MnPN 的解剖学、神经化学和电生理学特征。 计划的实验将有助于更全面地了解下丘脑机制在正常和紊乱的睡眠和觉醒状态中的作用。