MONOCYTE TISSUE FACTOR EXPRESSION IN DIABETES MELLITUS

糖尿病中单核细胞组织因子的表达

• 批准号: 6540698
• 负责人: JAMES A KATANCIK
• 金额: $ 10.79万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6540698
• 关键词: coagulation factor VII; enzyme linked immunosorbent assay; flow cytometry; gel mobility shift assay; gene expression; genetic transcription; glycoproteins; human subject; human tissue; inflammation; insulin dependent diabetes mellitus; interleukin 1; molecular pathology; monocyte; nuclear factor kappa beta; patient oriented research; phenotype; prostaglandin E; protein structure function; thromboplastin; tissue /cell culture; transcription factor; transfection; tumor necrosis factor alpha

PROPOSAL (Adapted from the applicant's abstract): The candidate is a new investigator with a clinical doctoral degree in dentistry and a basic science doctoral degree in oral biology. The objective of this proposal is to combine the candidate's clinical and basic science background so that he may independently and competently carry out and mentor patient-centered research in the future. A multi-disciplinary study within the environment of the General Clinical Research Center (GCRC) at the UTM is proposed. A fully integrated research career development plan includes training in epidemiology, biostatistics, and study design leading to matriculation from the UTM Master's in Epidemiology Program. The candidate will also receive training in experimental design and analysis of results, publication and presentation of results, and grantsmanship. The proposed interdisciplinary biomedical research project focuses on monocyte expression of tissue factor (TF), a cell surface-bound glycoprotein receptor with procoagulant and proinflammatory functions, in a systemic disease, diabetes mellitus, and an oral complication, periodontitis. The project includes direct patient contact: a dental examination and collection of a blood sample and complementary laboratory research of monocyte TF expression, the role of TF in the monocyte secretory phenotype, and the role of TF in monocyte transcriptional activity. Immediately the career development award will allow the candidate to broaden his knowledge in epidemiology, biostatistics, and research design while at the same time providing the opportunity to expand his research skills in molecular biology techniques and studies in transcription regulation. In the long-term the career development award will provide the candidate with the background necessary to carry out and mentor quality research in the rapidly expanding and exciting area of biomedical research examining the inter-relationship of systemic and oral diseases.

建议(改编自申请者的摘要)：候选人是一名新的 具有牙科和基础科学临床博士学位的调查员 口腔生物学博士学位。这项提议的目标是将 候选人的临床和基础科学背景，以便他可以 独立、胜任地开展和指导以患者为中心的研究 在未来。大环境下的多学科研究 建议在UTM建立综合临床研究中心(GCRC)。一个完整的 综合研究职业发展计划包括流行病学培训， 生物统计学和研究设计导致从UTM硕士的入学 在流行病学项目中。候选人还将接受以下方面的培训 实验设计和结果分析、出版和演示 结果，和恩赐精神。拟议的跨学科生物医学 研究项目集中在单核细胞表达组织因子(TF)这一细胞 具有促凝剂和促炎作用的表面结合糖蛋白受体 在系统性疾病、糖尿病和口腔并发症中的功能， 牙周炎。该项目包括与患者的直接接触：牙科 检验及采集血液样本及辅助化验室 单核细胞转铁蛋白的表达及其在单核细胞分泌中的作用研究 表型，以及转铁蛋白在单核细胞转录活性中的作用。 职业发展奖将立即允许应聘者拓宽 他在流行病学、生物统计学和研究设计方面的知识 同时提供了扩大他在分子方面的研究技能的机会 转录调控的生物技术和研究。从长远来看 职业发展奖将为候选人提供背景知识 有必要在迅速扩大的质量研究中开展和指导 以及令人兴奋的生物医学研究领域，研究了 全身和口腔疾病。