MONOCYTE TISSUE FACTOR EXPRESSION IN DIABETES MELLITUS

糖尿病中单核细胞组织因子的表达

• 批准号: 6735598
• 负责人: JAMES A KATANCIK
• 金额: $ 10.43万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2005-11-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6735598
• 关键词: coagulation factor VII; enzyme linked immunosorbent assay; flow cytometry; gel mobility shift assay; gene expression; genetic transcription; glycoproteins; human subject; human tissue; inflammation; insulin dependent diabetes mellitus; interleukin 1; molecular pathology; monocyte; nuclear factor kappa beta; patient oriented research; phenotype; prostaglandin E; protein structure function; thromboplastin; tissue /cell culture; transcription factor; transfection; tumor necrosis factor alpha

PROPOSAL (Adapted from the applicant's abstract): The candidate is a new investigator with a clinical doctoral degree in dentistry and a basic science doctoral degree in oral biology. The objective of this proposal is to combine the candidate's clinical and basic science background so that he may independently and competently carry out and mentor patient-centered research in the future. A multi-disciplinary study within the environment of the General Clinical Research Center (GCRC) at the UTM is proposed. A fully integrated research career development plan includes training in epidemiology, biostatistics, and study design leading to matriculation from the UTM Master's in Epidemiology Program. The candidate will also receive training in experimental design and analysis of results, publication and presentation of results, and grantsmanship. The proposed interdisciplinary biomedical research project focuses on monocyte expression of tissue factor (TF), a cell surface-bound glycoprotein receptor with procoagulant and proinflammatory functions, in a systemic disease, diabetes mellitus, and an oral complication, periodontitis. The project includes direct patient contact: a dental examination and collection of a blood sample and complementary laboratory research of monocyte TF expression, the role of TF in the monocyte secretory phenotype, and the role of TF in monocyte transcriptional activity. Immediately the career development award will allow the candidate to broaden his knowledge in epidemiology, biostatistics, and research design while at the same time providing the opportunity to expand his research skills in molecular biology techniques and studies in transcription regulation. In the long-term the career development award will provide the candidate with the background necessary to carry out and mentor quality research in the rapidly expanding and exciting area of biomedical research examining the inter-relationship of systemic and oral diseases.

建议（改编自申请人的摘要）：候选人是一个新的 拥有牙科临床博士学位和基础科学研究员 口腔生物学博士学位。 本提案的目的是将联合收割机 候选人的临床和基础科学背景，使他可以 独立和胜任地开展和指导以患者为中心的研究 在未来 一个多学科的研究环境中的 建议在UTM设立一般临床研究中心（GCRC）。 完全 综合研究职业发展计划包括流行病学培训， 生物统计学和研究设计导致从UTM硕士预科 流行病学项目。 候选人还将接受以下培训： 实验设计和结果分析，出版和介绍 结果，和granitarian。 建议的跨学科生物医学 研究项目集中在单核细胞表达组织因子（TF），一种细胞 具有促凝血和促炎作用表面结合糖蛋白受体 功能，在全身性疾病、糖尿病和口腔并发症中， 牙周炎 该项目包括直接接触病人： 血液样本的检查和采集以及辅助实验室 单核细胞TF表达的研究，TF在单核细胞分泌中的作用， 表型，以及TF在单核细胞转录活性中的作用。 立即职业发展奖将允许候选人扩大 他在流行病学、生物统计学和研究设计方面的知识， 同时提供扩大分子研究技能的机会 生物学技术和转录调控研究。 长期 职业发展奖将为候选人提供 在快速扩张的市场中开展和指导高质量的研究是必要的。 生物医学研究的一个令人兴奋的领域， 全身和口腔疾病。