Solid-State NMR Structural Studies of Beta-Amyloid

β-淀粉样蛋白的固态核磁共振结构研究

• 批准号: 6664336
• 负责人: NIKOLAUS Martin LOENING
• 金额: $ 3.56万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6664336
• 关键词: Alzheimer's disease; amyloid proteins; computer simulation; high performance liquid chromatography; intermolecular interaction; model design /development; molecular dynamics; neurofibrillary tangles; nuclear magnetic resonance spectroscopy; peptide chemical synthesis; physical model; protein purification; protein structure function; radiotracer; statistics /biometry; structural biology; technology /technique development

The long-term objective of this research is to attain a better understanding of the structure of fibrils composed of fl-amyloid peptides. These fibrils are the main component of the senile plaques found in patients with Alzheimer's disease and have been implicated as the toxic species in that disease. It is hoped that a better understanding of the structure of these fibrils and the peptides of which they are composed will lead to preventative and therapeutic treatments for Alzheimer's disease. This project will initially focus on a model peptide before moving on to a subsection of the fl-amyloid peptide and, eventually, the complete peptide. The main technique for determining the structure of fl- amyloid will be solid-state nuclear magnetic resonance spectroscopy. The further development of this technique that will occur ill the course of this research project is likely to extend the applicability of solid-state nuclear magnetic resonance for structural determination of biological molecules.

这项研究的长期目标是更好地了解由 fl-淀粉样肽组成的原纤维的结构。这些原纤维是阿尔茨海默病患者中发现的老年斑的主要成分，并且被认为是该疾病中的有毒物质。希望更好地了解这些原纤维的结构及其组成的肽将有助于预防和治疗阿尔茨海默病。该项目最初将重点关注模型肽，然后再转向 FL-淀粉样肽的一部分，并最终形成完整的肽。确定淀粉样蛋白结构的主要技术是固态核磁共振波谱。在本研究项目过程中，这项技术的进一步发展可能会扩展固态核磁共振在生物分子结构测定中的适用性。